Enriched Gamma Delta T Cell Compositions for Durable Coronavirus Immunity

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Solution Overview

Problem

Current treatments and vaccines for SARS-CoV-2 infections are inadequate due to high morbidity and mortality, and existing immunity is short-lived, necessitating new approaches for effective immune response elicitation.

Innovation Solution

Development of compositions enriched with Vgamma9 Vdelta2 (Vγ9Vδ2) T cells, specifically CD45RA−CD27− effector memory and CD45RA−CD27+ central memory cells, achieved through isolation, culturing with cytokines and bisphosphonates, and incubation with coronavirus spike protein antigens, to induce a robust immune response.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If existing treatments and vaccines are used for SARS-CoV-2 infections, then treatment is provided, but the treatments are inadequate due to high morbidity and mortality and short-lived immunity

Engineering Contradiction:

Improveeffectiveness of treatment and vaccineVSAvoidduration of immunity

Core Design Contradiction:

ReliabilityVSDuration of action of moving object

Solution Approach 1:

The patent changes the parameters of the immune response by using enriched Vγ9Vδ2 T cells with specific memory phenotypes (central and effector memory) that provide more durable and effective immunity compared to conventional vaccines. The composition is engineered to elicit both cellular and humoral responses with extended duration

Inventive Principle:

Principle #35Parameter changes

Solution Approach 2:

The invention creates a composite immune response by combining multiple components: enriched Vγ9Vδ2 T cells, cytokines (IL-2, IL-7, IL-15), and coronavirus spike protein antigens. This composite approach produces a more robust and long-lasting immune response than single-component vaccines

Inventive Principle:

Principle #40Composite materials

2Reliability

If enriched Vgamma9 Vdelta2 T cell compositions are developed, then robust immune response is elicited, but the complexity of cell isolation and culturing increases

Engineering Contradiction:

Improverobustness of immune responseVSAvoidcomplexity of cell composition preparation

Core Design Contradiction:

ReliabilityVSDevice complexity

Solution Approach 1:

The patent performs preliminary actions by pre-enriching and expanding Vγ9Vδ2 T cells ex vivo with specific memory phenotypes before administration. The cells are pre-conditioned with cytokines and antigen exposure to ensure they are primed and ready to elicit a robust immune response upon injection, thereby simplifying the in vivo process

Inventive Principle:

Principle #10Preliminary action

Solution Approach 2:

The enriched T cell composition acts as an intermediary that bridges the gap between conventional vaccines and the desired robust immune response. These engineered cells serve as living mediators that can directly interact with the immune system to produce both cellular and humoral responses with greater durability

Inventive Principle:

Principle #24Intermediary (Mediator)

Data Source

PatentUS20230263827A1Expanded memory subsets of gamma delta t cells for immunotherapy

Publication Date: 2023.08.24 GUMRUKCU SERHAT

AI summary

The present disclosure provides compositions comprising enriched gamma delta T cells and methods for generating and expanding the enriched gamma delta T cells. In certain embodiments, these enriched gamma delta T cells are used for eliciting an immune response in a subject for methods of treating and/or preventing a coronavirus infection.