Gamma Sterilized Dextran Solutions for Blood Separation
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Conventional blood cell isolation procedures require separate sterilization of dextran solutions, which are unstable to gamma irradiation, leading to molecular weight decomposition and increased costs and contamination risks.
Innovation Solution
Development of gamma stable dextran solutions with an initial molecular weight greater than 500 kD and the addition of ascorbic acid or its mineral salt, allowing for direct gamma sterilization while maintaining effective red blood cell aggregation performance.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If dextran solution is sterilized by gamma irradiation, then sterilization is achieved, but molecular weight decomposition occurs
Solution Approach 1:
The patent applies preliminary action by formulating dextran solutions with higher initial molecular weights (greater than 500 kD) before sterilization. This pre-positioning of higher molecular weight compensates for the expected degradation during gamma irradiation, ensuring that post-sterilization molecular weight remains sufficient for effective RBC aggregation (above 200 kD).
Solution Approach 2:
The patent changes the molecular weight parameter of the dextran from conventional lower weights to significantly higher weights (>500 kD initial, >200 kD after sterilization). This parameter change allows the dextran to withstand gamma irradiation-induced decomposition while maintaining functional effectiveness for blood cell separation.
2Reliability
If separate sterilization of dextran solution is performed, then sterilization is achieved, but manufacturing complexity and cost increase
Solution Approach 1:
The patent merges the sterilization of dextran solution with the sterilization of the blood processing system by integrating the dextran into a sterile container or closed system that can be sterilized together with the blood sample through gamma irradiation. This eliminates separate sterilization steps, reduces manufacturing complexity, and minimizes contamination risks during assembly.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The gamma sterilized dextran solutions enable seamless integration into the manufacturing process, reducing handling errors and costs while maintaining the molecular weight necessary for effective red blood cell sedimentation and nucleated cell recovery.
Implementation Method 1
sterilization of the kit which is accomplished by exposure to gamma irradiation at a dose between 20 and 50 kGy
Implementation Method 2
dextran in water is unstable to gamma irradiation resulting in severe molecular weight decomposition
Implementation Method 3
dextran as an aggregant to enhance the sedimentation of red blood cells from whole blood
Data Source
AI summary
Provided herein are kit for providing a gamma sterilized aqueous dextran solution that increase the efficiency of blood separation by allowing the dextran solution to be sterilized by exposure to gamma radiation while maintaining sufficient molecular weight to act as a red blood cell aggregate. Also provided are methods of use.