Gas Bubbling Sample Pretreatment Device for Amino Acid Analysis
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Solution Overview
Problem
High concentrations of organic solvents in reagents used for amino acid sample pretreatment can lead to sample loss and inefficient analysis due to sample adhesion to vessel surfaces and suboptimal separation conditions, requiring manual and time-consuming drying and re-dissolution processes.
Innovation Solution
A device with a vessel, reagent introduction, discharge, and gas supply parts that form gas bubbles in the reagent to promote solvent volatilization, reducing organic solvent concentration efficiently and preventing sample adhesion, utilizing a first gas supply for pressurization and a second gas supply for bubble formation in the reagent.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Loss of substance
If the amino acid sample is completely dried in a vessel, then the organic solvent concentration is reduced, but the amino acid precipitates and adheres to the inner surface of the vessel causing sample loss
Solution Approach 1:
The patent replaces the mechanical manual operation of drying and re-dissolving with an automated gas bubbling system. Gas bubbles are introduced into the reagent to promote solvent volatilization and enable automatic concentration adjustment, eliminating the need for manual drying and re-dissolution steps while preventing sample adhesion to vessel surfaces.
Solution Approach 2:
The patent utilizes gas bubbling (pneumatic method) to promote solvent volatilization. By introducing gas bubbles into the reagent containing the amino acid sample, the system achieves solvent removal and concentration adjustment without complete drying, preventing sample precipitation and adhesion while automating the process.
2Measurement precision
If concentration of the organic solvent is high, then the reagent is effective for dissolving amino acid samples, but the amino acid sample cannot be favorably separated in the high-performance liquid chromatograph
Solution Approach 1:
The patent dynamically adjusts the organic solvent concentration parameter by introducing gas bubbles to promote volatilization. This allows the system to optimize the solvent concentration for separation quality in real-time, achieving favorable amino acid separation while maintaining efficient analysis throughput by automating the concentration adjustment process.
3Productivity
If manual drying and re-dissolution processes are performed, then organic solvent concentration is adjusted, but the work takes time and labor extending analysis duration
Solution Approach 1:
The patent replaces manual mechanical operations with an automated gas bubbling system that promotes solvent volatilization. This automation eliminates the time-consuming manual drying and re-dissolution steps, significantly improving analysis efficiency while reducing labor requirements.
Solution Approach 2:
The gas bubbling process enables continuous solvent volatilization and concentration adjustment without interrupting the analysis workflow. Unlike manual drying and re-dissolution which require stopping the process, the automated system maintains continuous operation, reducing total analysis time and improving productivity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method effectively lowers organic solvent concentration, prevents sample loss, and streamlines the analysis process by promoting solvent volatilization through gas bubbles, enhancing the efficiency and accuracy of amino acid sequence analysis.
Implementation Method 1
gas bubbles are formed in the reagent and volatilization of the organic solvent in the reagent is promoted by the gas bubbles
Implementation Method 2
The first gas supply part supplies a gas into the vessel and thus pressurizes the interior of the vessel
Data Source
Figure 1
Figure 2
Figure 3A
AI summary
A sample is dissolved in a reagent 4 containing an organic solvent in a conversion vessel 11. A gas is supplied from a first gas supply part into the conversion vessel 11 via a reagent introduction tube 17, and thus the interior of the conversion vessel 11 is pressurized. A gas is supplied from a second gas supply part into the reagent 4 in the conversion vessel 11 via a reagent discharge tube 18, and thus gas bubbles 41 are formed in the reagent 4.