Gas-Permeable Containers for TIL Expansion
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Solution Overview
Problem
Adoptive cell therapy using tumor infiltrating lymphocytes (TIL) for cancer treatment is hindered by complex and labor-intensive cell processing techniques, leading to technical, regulatory, and logistical challenges.
Innovation Solution
A method involving the use of gas permeable containers for culturing and expanding TIL using irradiated allogeneic or autologous feeder cells, which simplifies the process, reduces microbial contamination risk, and is more suitable for clinical use by minimizing equipment and reagent requirements.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional cell processing techniques are used for TIL expansion, then TIL can be obtained for therapy, but the process becomes complex and labor-intensive with technical and regulatory challenges
Solution Approach 1:
The patent extracts and eliminates unnecessary complex processing steps from conventional TIL expansion methods. By using a simplified culture system with gas-permeable containers and standardized protocols, the invention removes redundant operations while maintaining effective TIL expansion, thereby reducing both device complexity and processing time.
Solution Approach 2:
The invention changes key culture parameters including using gas-permeable containers to optimize oxygen and carbon dioxide exchange, implementing specific feeder cell ratios, and establishing defined culture conditions. These parameter changes enable efficient TIL expansion through a simpler, more standardized process that reduces technical complexity.
2Reliability
If conventional cell processing techniques are used, then TIL expansion can be achieved, but microbial contamination risk increases
Solution Approach 1:
The patent employs disposable gas-permeable culture containers that are pre-sterilized and designed for single use. This eliminates the need for complex sterilization procedures and reduces microbial contamination risk, as each container is used once and then discarded, preventing cross-contamination while simplifying the overall processing procedure.
3Ease of manufacture
If simplified gas permeable container method is used, then processing complexity is reduced, but TIL expansion capability must be maintained
Solution Approach 1:
The patent uses irradiated allogeneic or autologous feeder cells as intermediaries to stimulate TIL proliferation in the simplified gas-permeable container system. These feeder cells provide necessary growth signals and cytokines that enable robust TIL expansion despite the reduced complexity of the culture system, thereby maintaining productivity while improving ease of manufacture.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method enables the efficient expansion of TIL, making adoptive cell therapy more accessible and effective, allowing for the treatment of a wider range of cancers and increasing the number of patients who can benefit from this therapy.
Implementation Method 1
culturing and expanding TIL in gas permeable containers
Data Source
AI summary
An embodiment of the invention provides a method of promoting regression of cancer in a mammal comprising obtaining a tumor tissue sample from the mammal; culturing the tumor tissue sample in a first gas permeable container containing cell medium therein; obtaining tumor infiltrating lymphocytes (TIL) from the tumor tissue sample; expanding the number of TIL in a second gas permeable container containing cell medium therein using irradiated allogeneic feeder cells and/or irradiated autologous feeder cells; and administering the expanded number of TIL to the mammal. Methods of obtaining an expanded number of TIL from a mammal for adoptive cell immunotherapy are also provided.