Integrated Gene Detection Kit with Interconnected Cavities
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Solution Overview
Problem
Current gene detection methods are complex and prone to sample pollution due to the need for multiple devices and steps, which can affect detection accuracy and efficiency.
Innovation Solution
A gene detection method and device with interconnected separation cavities in a single kit, using a plunger to sequence lysing, washing, and reaction steps with magnetic beads and enzymes, allowing for optical detection without secondary pollution, facilitated by electromagnetic control and a compact design.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If multiple devices are used for different detection steps, then the detection can be performed with specialized equipment for each step, but the detection process becomes complicated and sample pollution occurs
Solution Approach 1:
The patent combines multiple detection steps (lysis, washing, amplification, detection) into a single integrated device with interconnected cavities. The sample remains in the same system throughout all steps, eliminating the need to transfer between devices and preventing cross-contamination while maintaining specialized functionality for each step.
Solution Approach 2:
The integrated device is divided into functionally independent cavities (lysis cavity, washing cavity, amplification cavity, detection cavity) that can operate independently but are connected to allow continuous sample processing. Each cavity is optimized for its specific function while maintaining physical connection to prevent sample transfer.
2Ease of manufacture
If the sample is transferred to different carriers for different steps, then each step can be optimized independently, but sample pollution occurs and detection accuracy is affected
Solution Approach 1:
The patent maintains the sample in a single continuous system throughout all detection steps, eliminating transfers between carriers. The interconnected cavity design allows the sample to flow through different functional zones without physical removal or transfer, preventing pollution while maintaining functional optimization.
3Productivity
If multiple devices are used for gene detection, then each device can be specialized for its function, but the detection efficiency decreases due to complicated procedures
Solution Approach 1:
The patent integrates multiple detection functions into a single device with interconnected cavities, allowing the sample to proceed through all steps without transfer. This streamlined approach eliminates the complexity of coordinating multiple devices and procedures, significantly improving detection efficiency and operational simplicity.
4Adaptability or versatility
If sample transfer between devices is performed, then different specialized equipment can be utilized, but pollution is introduced and affects detection accuracy
Solution Approach 1:
The patent creates an integrated system where all detection steps occur within connected cavities without sample transfer to external devices. This eliminates the pollution risk associated with sample transfer while maintaining specialized functional zones for each detection step within the same sealed system.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach simplifies gene detection, improves efficiency, and enhances accuracy by integrating all steps in one device, reducing the need for multiple devices and minimizing sample handling, thereby preventing pollution and improving detection precision.
Implementation Method 1
controlling magnetic beads in the kit to drive the sample to be tested to pass through the each separation cavity in sequence by an electromagnetic control method
Implementation Method 2
an enzyme required for the reaction is provided in the plunger hole of the plunger, and when the plunger hole of the plunger is aligned with the separation cavity, the enzyme automatically falls into the reaction solution due to a gravity of the enzyme
Data Source
AI summary
A gene detection method, a gene detection kit, and a gene detection device, including the following steps: providing a plurality of separation cavities on a kit, using a plunger to separate adjacent separation cavities, and respectively providing a lysate solution, a washing solution and a reaction solution in the separation cavities; when detecting a sample, pushing each plunger to align a plunger hole of the plunger with the separation cavity, thereby making the separation cavities interconnected; then, controlling magnetic beads in the kit to drive the sample to be tested to pass through the separation cavities in sequence by an electromagnetic control method, carrying out a lysing, a washing and a reaction in sequence; and finally, performing a optical detection on a gene in the reaction solution from outside.