Gene Detection Kit Piston Cylinder Channel Alignment
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Solution Overview
Problem
Conventional gene detection processes face challenges with cross-contamination and high costs due to the need for multiple instrument steps and reagent transfers, leading to inaccurate results and safety hazards.
Innovation Solution
A gene detection kit with a piston cylinder and reagent cavities that allows for centralized, enclosed nucleic acid extraction, eliminating the need for external transfers by using a movable piston cylinder to alternate communication between channels, reducing the risk of cross-contamination and manufacturing complexity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If multiple instrument steps and reagent transfers are used in conventional gene detection, then the detection process can be completed, but cross-contamination risk increases and detection accuracy decreases
Solution Approach 1:
The patent merges multiple separate instrument steps into a single integrated extraction device. The device combines sample lysis, magnetic bead separation, washing, and elution functions within one enclosed system, eliminating the need for multiple instrument transfers and reducing cross-contamination risk between samples and reagents.
Solution Approach 2:
The patent introduces magnetic beads as an intermediary substance to facilitate nucleic acid extraction. The magnetic beads bind to nucleic acids and can be selectively separated using a magnetic field, enabling efficient purification without direct contact between different reagent systems and minimizing contamination opportunities.
2Ease of manufacture
If multiple instrument steps are used in conventional gene detection, then the extraction process can be completed, but the complexity of the system increases
Solution Approach 1:
The patent integrates multiple extraction functions (lysis, separation, washing, elution) into a single device structure with one piston cylinder and multiple reagent cavities. This merging approach reduces the number of separate instruments needed and simplifies the overall system while maintaining complete extraction capability.
Solution Approach 2:
The extraction device is designed as a universal multi-functional system where a single piston cylinder can perform multiple operations (lysing, washing, eluting) by sequentially communicating with different reagent cavities. This multi-functionality eliminates the need for separate specialized instruments for each extraction step.
3Productivity
If multiple reagent transfers are performed in conventional gene detection, then the extraction steps can be completed, but the time required for the process increases
Solution Approach 1:
The patent enables continuous extraction processing by maintaining the sample within a single enclosed system throughout all extraction steps. The piston sequentially communicates with different reagent cavities without removing the sample, eliminating transfer time and maintaining continuous productive action throughout the extraction process.
Solution Approach 2:
The patent employs a dynamic piston mechanism that can move between different positions to selectively communicate with various reagent cavities. This dynamic operation allows rapid sequential execution of lysis, separation, washing, and elution steps without the time loss associated with manual transfers between static instruments.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution enhances the accuracy of gene detection results, reduces the risk of contamination, and lowers manufacturing and operational costs by simplifying the extraction process within an enclosed system.
Implementation Method 1
a piston, wherein the piston is movably provided in the piston cavity along an axial direction of the piston cylinder
Implementation Method 2
the piston cylinder can move relative to the kit body, so that the plurality of second channels are alternately in communication with the first channel
Data Source
AI summary
Provided are a gene detection kit and a gene detection device. The gene detection kit includes a kit body, a piston cylinder, and a piston. The kit body has an accommodating cavity and a plurality of reagent cavities. The piston cylinder is provided in the accommodating cavity, and the piston cylinder has a piston cavity. The piston is movably provided in the piston cavity along an axial direction of the piston cylinder. A first channel in communication with the piston cavity is provided on an outer circumferential surface of the piston cylinder, a plurality of second channels are provided on an inner wall of the accommodating cavity, each of the second channels is in corresponding communication with one of the reagent cavities, and the piston cylinder can move relative to the kit body, so that the plurality of second channels are alternately in communication with the first channel.