Gene Set Modulation for Mammalian Tissue Regeneration

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Solution Overview

Problem

Current methods for tissue regeneration in mammals are inadequate, particularly in addressing trauma and degenerative diseases, as the molecular mechanisms for regeneration in animals like planaria and amphibians are not understood in humans, and existing treatments are ineffective.

Innovation Solution

The use of specific gene sets, including PCDHB2, PCDHB17, Nbla10527, RAB3IP, DLX1, DRD11P, FOXD1, LOC728755, AFF3, F2RL2, MN1, CBCAQHO3, LOC791120, SIX1, OXTR, and WSB1, or their inhibitors, to enhance or inhibit tissue regeneration by modulating gene expression.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If stem cell transplantation is used to treat tissue damage, then tissue regeneration is improved, but immune rejection and transplant compatibility issues worsen

Engineering Contradiction:

Improvetissue regeneration effectivenessVSAvoidimmune rejection

Core Design Contradiction:

ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent extracts and removes the MHC class I gene from the stem cell genome, effectively taking out the problematic antigen-presenting component that triggers immune rejection. This extraction approach allows the stem cells to retain their therapeutic regenerative capabilities while eliminating the specific genetic element responsible for immune system recognition and rejection

Inventive Principle:

Principle #2Taking out (Extraction)

Solution Approach 2:

The patent converts the harmful immune recognition mechanism into a benefit by selectively removing only the MHC class I gene while preserving other essential cellular functions. The resulting stem cells become universally compatible donors, transforming the previously harmful immune rejection response into a universally accepted therapeutic solution

Inventive Principle:

Principle #22Blessing in disguise (Convert harm into benefit)

2Adaptability or versatility

If MHC class I gene is removed from stem cells, then transplant compatibility is improved, but cell surface antigen presentation and immune system interaction worsen

Engineering Contradiction:

Improvetransplant compatibilityVSAvoidimmune system interaction

Core Design Contradiction:

Adaptability or versatilityVSReliability

Solution Approach 1:

The patent applies local quality modification by selectively altering only the MHC class I gene expression in specific regions of the cell (cell surface), while leaving the rest of the cell's immune interaction capabilities intact. This localized genetic modification allows the cell to maintain appropriate immune system interactions for therapeutic function while achieving universal transplant compatibility

Inventive Principle:

Principle #3Local quality

Solution Approach 2:

Instead of trying to add or modify multiple genes to achieve compatibility, the patent inverts the approach by removing the specific gene (MHC class I) that causes incompatibility. This subtraction-based strategy simplifies the genetic modification process and achieves universal compatibility more effectively

Inventive Principle:

Principle #13The other way round (Inversion)

3Productivity

If gene expression is modified to enhance regeneration, then tissue repair capability is improved, but genetic stability and potential tumorigenesis worsen

Engineering Contradiction:

Improvetissue repair capabilityVSAvoidgenetic stability

Core Design Contradiction:

ProductivityVSReliability

Solution Approach 1:

The patent applies partial action by modifying only the specific MHC class I gene expression rather than making comprehensive genetic changes to enhance regeneration. This targeted approach achieves the desired therapeutic effect while minimizing disruptions to overall genetic stability and reducing potential risks of tumorigenesis

Inventive Principle:

Principle #16Partial or excessive action

Data Source

PatentUS12492400B2Compositions and methods for induced tissue regeneration in mammalian species

Publication Date: 2025.12.09 REVERSE BIOENGINEERING INC

AI summary

Aspects of the present invention include methods and compositions related to the modulation of molecules regulating the regenerative potential of cells and tissues in the embryonic state and the loss thereof in later fetal and adult stages of development. The methods and compositions have uses in research in stem cell biology and in increasing regenerative potential in fetal and adult tissues otherwise incapable of regeneration.