Genome Amplification Module With Branch Space for Uniform Extract Distribution
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Solution Overview
Problem
Conventional genome amplification modules face issues such as reagent leakage, cross-contamination, and inaccurate detection due to uneven extract distribution, which are exacerbated by vibration and capillary phenomena.
Innovation Solution
A genome extraction device with a dual-chamber structure, safety clips, and specific protrusion members to prevent leakage and cross-contamination, along with a design that ensures equal extract distribution and bubble minimization in the amplification module.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Device complexity
If a single chamber structure is used to accommodate reagents, then device complexity is reduced, but reagent leakage occurs and reliability deteriorates
Solution Approach 1:
The chamber is divided into multiple separate chambers (first chamber, second chamber, third chamber) with distinct sealing mechanisms. Each chamber can be independently sealed and controlled, preventing reagent leakage while maintaining manageable device complexity through modular design.
2Device complexity
If vibration resistance is not considered in design, then device complexity is reduced, but cross-contamination occurs between chambers, worsening reliability
Solution Approach 1:
The sealing members are designed with elastic properties and pre-positioned to accommodate vibrations before they occur. The elastic sealing members can deform to maintain sealing contact during vibrations, preventing cross-contamination without requiring complex active vibration protection systems.
3Ease of operation
If extract is injected without considering gravity effects, then injection process is simplified, but uneven extract distribution occurs in receptacles, worsening measurement precision
Solution Approach 1:
The receptacles are positioned asymmetrically within the amplification module, with varying distances from the injection port. This asymmetric arrangement, combined with the branch space design, compensates for gravity-induced flow variations, ensuring that each receptacle receives a uniform amount of extract despite different gravitational effects during injection.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The device effectively prevents reagent leakage and cross-contamination, ensures uniform extract distribution, and enhances detection accuracy by minimizing bubble interference.
Implementation Method 1
a first sealing member S1 and a second sealing member S2 which are respectively made of an elastic material and elastically seal the first opening and the second opening
Implementation Method 2
a first protrusion member 111 and a second protrusion member 112 which are respectively formed on the cover 300 and the outer chamber 100 and tear the first sealing member S1 and the second sealing member S2
Implementation Method 3
solves the problem of cross-contamination between reagents by a capillary phenomenon occurring through a space between double chambers
Data Source
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AI summary
The present invention relates to a genome amplification module having a branch space adjacent to an inlet through which an extract is introduced, the genome amplification module in which a branch space is formed in a portion adjacent to an inlet and is wider and deeper than an extract moving passage so that the extract can be simultaneously injected along each extract moving passage after the extract is filled in the branch space.