Genome Amplification Module With Branch Space for Uniform Extract Injection
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Solution Overview
Problem
Conventional genome amplification modules face issues such as reagent leakage, cross-contamination, inaccurate detection due to extract mixing, and uneven injection volumes, which affect the reliability and accuracy of nucleic acid analysis.
Innovation Solution
The genome amplification module features a dual-chamber design with sealed inner and outer chambers, a safety clip to prevent reagent leakage, a branch space adjacent to the inlet for uniform extract distribution, and separate receptacles for different probes, along with controlled gas and extract passages to minimize bubbles and ensure accurate detection.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If extracts are injected into multiple receptacles simultaneously, then productivity is improved, but inaccurate detection results occur due to extract mixing between receptacles
Solution Approach 1:
The module is divided into multiple independent receptacles, each with its own dedicated extract moving passage. This segmentation prevents extract mixing between different receptacles while allowing simultaneous injection into all receptacles, thus maintaining both high productivity and accurate detection results for each sample
2Device complexity
If a single chamber contains all reagents, then device complexity is reduced, but reagent leakage occurs
Solution Approach 1:
The reagent system is segmented into multiple independent chambers, each containing specific reagents required for different amplification reactions. This segmentation prevents cross-contamination and leakage between chambers while maintaining overall system reliability
3Ease of operation
If extracts are injected without controlled passages, then ease of operation is improved, but uneven injection volumes occur due to gravity
Solution Approach 1:
Extract moving passages serve as intermediaries between the extract reservoir and the receptacles. These passages are designed with specific geometries and orientations that control extract flow, ensuring uniform injection volumes into each receptacle while maintaining simple operation through automated injection
4Device complexity
If gas passages are not separated from extract passages, then device complexity is reduced, but bubble interference affects detection accuracy
Solution Approach 1:
The module contains separate gas moving passages and extract moving passages that are spatially distinct. This segmentation allows gas to be vented through dedicated pathways without interfering with extract flow and detection, thereby maintaining high detection accuracy while managing system complexity
Data Source
AI summary
The present invention relates to a genome amplification module having a branch space adjacent to an inlet through which an extract is introduced, the genome amplification module in which a branch space is formed in a portion adjacent to an inlet and is wider and deeper than an extract moving passage so that the extract can be simultaneously injected along each extract moving passage after the extract is filled in the branch space.