Genome Installation into Cell-Like Systems
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Solution Overview
Problem
Current methods for altering cellular genomes and membranes lack the ability to fully control and tailor the genomic and cellular environment, limiting experimental and bioengineering capabilities.
Innovation Solution
A method for installing a genome into a cell or cell-like system using naturally occurring, manmade, or hybrid nucleic acid sequences, accompanied by stabilizing materials, allowing for the design of cellular and membrane components, enabling the creation of cells with novel functions and capabilities.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If genomes are extracted from cells for transfer, then the genome can be installed into recipient cells or cell-like systems, but the genome may suffer damage during extraction and handling
Solution Approach 1:
The genome is extracted and prepared in advance, stabilized with proteins and polycations, and stored in a protective state before transfer. This preliminary preparation ensures the genome remains intact and ready for installation into recipient cells or cell-like systems without damage during handling.
2Ease of operation
If stabilizing materials are added to the genome, then the genome becomes more amenable to transfer, but the complexity of the system increases
Solution Approach 1:
Proteins and polycations are introduced as intermediary substances that facilitate genome transfer. These stabilizing materials act as mediators between the genome and the transfer process, protecting the genome and enabling its installation into recipient cells or cell-like systems without requiring complex equipment or procedures.
3Adaptability or versatility
If the genome configuration is modified (relaxed, supercoiled, or linearized), then the genome can be more easily introduced into recipient cells, but the natural chromosomal structure is altered
Solution Approach 1:
The physical state of the genome is temporarily modified by changing parameters such as supercoiling or linearization to facilitate introduction into recipient cells or cell-like systems. After successful installation, the genome can regain its natural chromosomal structure through cellular processes, thus achieving both easy introduction and structural stability.
4Manufacturing precision
If ghost cells are used as recipient systems, then the naturally occurring genome is eliminated for cleaner installation, but additional processing steps are required to create the ghost cells
Solution Approach 1:
The naturally occurring genome is extracted and removed from the recipient cell to create a ghost cell. This extraction process eliminates interfering genetic material, allowing for precise installation of the desired genome into the recipient cell or cell-like system without contamination or complexity from existing genomic elements.
Data Source
AI summary
A method is provided for introducing a genome into a cell or cell-like system. The introduced genome may occur in nature, be manmade with or without automation, or may be a hybrid of naturally occurring and manmade materials. The genome is obtained outside of a cell with minimal damage. Materials such as a proteins, RNAs, polycations, nucleoid condensation proteins, or gene translation systems may accompany the genome. The genome is installed into a naturally occurring cell or into a manmade cell-like system. A cell-like system or synthetic cell resulting from the practice of the provided method may be designed and used to yield gene-expression products, such as desired proteins. By enabling the synthesis of cells or cell-like systems comprising a wide variety of genomes, accompanying materials and membrane types, the provided method makes possible a broader field of experimentation and bioengineering than has been available using prior art methods.