Genome Selection Using Nucleic Acid Probe Hybridization
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Solution Overview
Problem
Current methods for selecting germ line genomes lack efficiency in identifying and isolating specific genetic characteristics, particularly for applications in fertilization, where targeted genetic traits for disease resistance, milk production, or egg laying are desired.
Innovation Solution
The development of systems and methods that include hybridization-based, chromatin decondensation-based, and spermatid subtractive determination methods for selecting male and female germ line genomes, allowing for the identification and isolation of genomes with specific genetic characteristics through nucleic acid sequencing and probe hybridization.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If traditional methods are used for selecting germ line genomes, then the process is simpler, but the efficiency in identifying and isolating specific genetic characteristics is low
Solution Approach 1:
The patent introduces nucleic acid probes as intermediary molecules that specifically bind to target genetic sequences. These probes act as mediators between the germ line genomes and the detection system, enabling efficient identification of specific genetic characteristics without requiring complex direct analysis methods
Solution Approach 2:
The patent replaces traditional mechanical or manual methods of genetic analysis with molecular hybridization techniques. By using nucleic acid probe binding reactions instead of physical separation or manual inspection methods, the system achieves higher efficiency in identifying genetic characteristics
2Measurement precision
If hybridization-based methods are used, then measurement precision of genetic characteristics improves, but the complexity of the detection process increases
Solution Approach 1:
The patent applies local quality by designing probes with specific sequences that target only particular genetic regions of interest. Each probe is tailored to bind to a specific nucleic acid sequence, enabling precise identification of desired genetic characteristics while ignoring other genomic regions
Solution Approach 2:
The patent utilizes parameter changes in the form of temperature control and salt concentration adjustment to optimize probe hybridization conditions. By carefully controlling these parameters, the system achieves high measurement precision in detecting genetic characteristics while managing the complexity of the hybridization process
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables the precise selection and isolation of germ line genomes based on genetic characteristics, enhancing the potential for improved offspring traits such as disease resistance and productivity in agricultural contexts.
Implementation Method 1
hybridization-based, chromatin decondensation-based, and spermatid subtractive determination methods for selecting male and female germ line genomes, allowing for the identification and isolation of genomes with specific genetic characteristics through nucleic acid sequencing and probe hybridization
Data Source
AI summary
Systems, methods, compositions and apparatus relating to genome selection are disclosed.