Genomic Analysis Device with Automated Liquid Handling
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Solution Overview
Problem
Current genomic analysis methods, particularly in avian gender sorting, are labor-intensive and require multiple instruments and manual processes, limiting throughput and efficiency.
Innovation Solution
A high-throughput genomic analysis system utilizing composite liquid cells (CLCs) with a thermal chip module, robotically controlled liquid handler, and optical interrogation station, enabling automated processing and analysis of multiple samples in a single device.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If manual processes and multiple instruments are used for genomic analysis, then operational flexibility is maintained, but productivity and throughput are limited
Solution Approach 1:
The patent combines multiple separate instruments (liquid handler, thermal cycler, plate reader, PCR system) into a single integrated automated system. The system uses a unified robotic liquid handling platform that can perform sample preparation, nucleic acid extraction, PCR amplification, and detection within one device, eliminating the need for manual transfer between multiple instruments and significantly increasing throughput.
Solution Approach 2:
The automated system is designed with multi-functional capabilities, where a single robotic platform can perform diverse genomic analysis tasks including sample dispensing, reagent addition, thermal cycling, and optical detection. This universal platform handles various sample types and assay protocols, replacing multiple specialized instruments while maintaining operational flexibility.
2Productivity
If automated systems are implemented, then productivity increases, but device complexity and initial setup requirements increase
Solution Approach 1:
The automated system is designed to operate with minimal human intervention once programmed. The robotic liquid handler automatically performs sample preparation, reagent dispensing, and plate manipulation without continuous manual guidance. The system includes self-calibration and self-diagnostic capabilities, reducing the need for complex operational procedures and making the system easier to use despite its advanced automation.
3Measurement precision
If multiple instruments are used, then measurement precision can be maintained, but loss of time due to manual processes increases
Solution Approach 1:
The integrated automated system enables continuous processing of samples without the interruptions inherent in manual multi-instrument workflows. The robotic platform continuously performs sample preparation, transfers samples to thermal cycling plates, executes PCR reactions, and immediately proceeds to detection, eliminating idle time between steps. This continuous operation maintains measurement precision while dramatically reducing total processing time compared to sequential manual operations.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The system significantly increases genomic analysis throughput and efficiency by automating sample handling, thermal cycling, and detection, reducing manual intervention and reagent consumption while providing accurate genetic characterization.
Implementation Method 1
The method relies on thermal cycling, DNA polymerase, primers (short DNA fragments complimentary to the targeted region of interest). Thermal cycling consists of repeated cycles of heating and cooling of the reaction for DNA melting and enzymatic replication of the DNA.
Implementation Method 2
The replicated DNA is detectable by many methods, the most common being through the use of fluorescently labeled probes that are specific to each allele.
Data Source
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AI summary
Aspects of the present disclosure include a complete genomic analysis device and methods of use thereof for high throughput genomic analysis of biological samples.