Germinative Growth Length Assessment via Image Analysis

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Solution Overview

Problem

Current methods for assessing the effect of chemical compounds on germinative units, such as fungi spores, are limited in accurately determining the vigor of these units, which is crucial for seed germination and growth, as they do not effectively differentiate between viability and vigor impacts.

Innovation Solution

A computer-implemented method that processes images of germinative units to identify and measure the length of germinative growths, calculating an average length to determine vigor, which correlates with the ability of units to germinate and grow under sub-optimum conditions, using techniques like image thresholding, clustering, and morphological closing to distinguish between growths and background.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Measurement precision

If the viability method (conidia germination method) is used to assess the effect of chemical compounds on germinative units, then the percentage of viable conidia can be determined, but the method cannot accurately differentiate between viability and vigor impacts

Engineering Contradiction:
Improveassessment accuracyVSAvoiddifferentiation capability
Core Design Contradiction:
Measurement precisionVSLoss of information

Solution Approach 1:

The patent segments the assessment process into two distinct measurement components: viability assessment (germination capability) and vigor assessment (growth rate measurement). By separating these previously conflated measurements, the system can independently evaluate each aspect and provide differentiated insights into chemical compound effects on germinative units.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent introduces a new measurement dimension by capturing and analyzing image data of germinative growths. This adds a spatial and visual dimension to the traditional viability assessment, enabling vigor evaluation through growth rate measurements that were previously unavailable in standard germination methods.

Inventive Principle:
Principle #17Another dimension (Dimensionality change)

2Measurement precision

If manual assessment methods are used to evaluate germinative growth, then detailed observation is possible, but the process is time-consuming and labor-intensive

Engineering Contradiction:
Improveobservation detailVSAvoidassessment duration
Core Design Contradiction:
Measurement precisionVSLoss of time

Solution Approach 1:

The patent creates digital copies of germinative growths through image capture. These image copies serve as permanent records that can be analyzed automatically by computer algorithms, replacing manual measurement while preserving detailed observational capabilities. The digital copies enable both precise measurement and efficient processing.

Inventive Principle:
Principle #26Copying

Solution Approach 2:

The patent replaces manual mechanical measurement processes with automated image processing and computer analysis. The system uses algorithms to automatically detect, measure, and calculate growth rates from images, substituting human observation and measurement with computational processes that are both precise and rapid.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

3Productivity

If image processing is used to identify and measure germinative growths, then automated assessment is achieved, but challenges arise in distinguishing growths from background and handling image variability

Engineering Contradiction:
Improveassessment speedVSAvoidimage processing complexity
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

The patent adjusts image processing parameters such as threshold values, contrast enhancement levels, and region-of-interest definitions to optimize the distinction between germinative growths and background. By dynamically tuning these parameters based on image characteristics, the system maintains high automated assessment capability while managing processing complexity.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The patent introduces intermediate processing steps including image preprocessing, segmentation, and feature extraction that serve as mediators between raw image data and final vigor assessment. These intermediary processes simplify the overall task by breaking down complex image analysis into manageable stages, reducing the computational burden while maintaining accuracy.

Inventive Principle:
Principle #24Intermediary (Mediator)

Data Source

PatentUS20230298195A1Computer implemented method for assessing the growth of germinative growths from germinative units
Publication Date: 2023.09.21 CRODA INT PLC
  • US20230298195A1 patent drawing
  • US20230298195A1 patent drawing
  • US20230298195A1 patent drawing

AI summary

A computer implemented method for assessing the growth of germinative units, the method comprising the steps of: processing an image of a sample comprising germinative units to identify at least one germinative growth, which is a growth germinating from a germinative unit, present in the image and determine the length of the at least one identified growth; and calculating an image average length of the at least one determined length.