Buffered Extraction Medium for GI Protein Dilution
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Solution Overview
Problem
Existing methods for determining protein concentrations in gastrointestinal tract samples, particularly fecal samples, face challenges with low recovery rates and instability of protein extracts, especially at higher dilutions, leading to inaccurate and unreliable results.
Innovation Solution
A method involving a dilution of the sample in a buffered aqueous extraction medium ranging from 1:100 to 1:10,000, which enhances protein yield, stability, and accuracy, allowing for more reliable detection of protein concentrations and improved disease monitoring.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If the sample is diluted with buffered aqueous extraction medium at higher dilutions (1:50 or higher), then the protein extraction yield increases, but the stability of protein extracts decreases leading to inaccurate results
Solution Approach 1:
The patent changes the dilution parameter from conventional 1:50 or higher to a specific range of 1:10 to 1:50 (optimally 1:20 to 1:40), which resolves the contradiction by finding the optimal balance point where sufficient protein yield is achieved while maintaining extract stability and accuracy
2Measurement precision
If the sample is diluted at lower concentrations (1:10 to 1:50), then the stability and accuracy of protein determination improves, but the protein yield may be insufficient for reliable detection
Solution Approach 1:
The patent optimizes the dilution parameter to the range of 1:10 to 1:50, which provides the ideal balance between maintaining measurement precision and achieving sufficient protein yield for reliable detection
3Ease of operation
If protein extracts are stored at elevated temperatures, then shipping and storage logistics are simplified, but the stability of protein extracts deteriorates
Solution Approach 1:
The optimized extraction method creates a buffer effect that protects protein stability during storage and shipping at elevated temperatures, allowing practical storage at 2-8°C without immediate freezing requirements
Solution Approach 2:
The specific dilution range of 1:10 to 1:50 creates optimal extraction conditions that enhance protein stability, allowing storage at 2-8°C for extended periods without degradation
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method provides higher and more stable protein extraction, leading to more accurate diagnostic results and better monitoring of diseases, with improved stability of protein extracts even at elevated temperatures, reducing the need for express shipping and cooling.
Implementation Method 1
a dilution of the sample in the buffered aqueous extraction medium in a range of 1:100 to 1:10,000 is obtained
Data Source
AI summary
The present invention concerns a method for determining the concentration of a protein in a gastrointestinal (GI) tract sample taken from a human or an animal. The present invention is characterized by the feature that a dilution of the sample in the buffered aqueous extraction medium in a range of 1:100 to 1:10,000 is obtained. The present invention leads to a significant improvement of the technical situation, and provides a simple, sensitive and specific determination tool of proteins in GI tract samples. The determination of proteins, e.g. calprotectin, elastases or hemoglobin, in GI tract samples leads to more accurate and reproducible results.