Ginger Enzyme Collagen Peptide Production for DPP-4 Inhibition
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Solution Overview
Problem
Current methods for producing tripeptides represented by X-Hyp-Gly are inefficient and costly due to the need for bacterial collagenase, which cleaves the wrong peptide bond, and require multi-step enzymatic processes, making industrial production challenging.
Innovation Solution
A method involving the use of ginger rhizome-derived enzymes to degrade collagen or gelatin, generating peptides like X-Hyp-Gly and X-Pro-Gly in a one-step reaction, with optional addition of glutathione-containing yeast extract and pH adjustment, to produce a collagen peptide composition with high DPP-4 inhibitory activity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Manufacturing precision
If bacterial collagenase is used to degrade collagen, then Gly-X-Y type peptides can be obtained, but the peptide bond between Hyp and Gly is cleaved, preventing formation of X-Hyp-Gly tripeptides
Solution Approach 1:
The invention changes the enzyme selection parameter from bacterial collagenase to plant-derived protease (zingibain from ginger rhizome). This parameter change fundamentally alters the cleavage specificity, enabling production of X-Hyp-Gly tripeptides that cannot be obtained with bacterial collagenase alone.
Solution Approach 2:
The invention introduces plant-derived protease as an intermediary enzyme that mediates the degradation of collagen to produce X-Hyp-Gly tripeptides. This intermediary enzyme bridges the gap between collagen substrate and desired tripeptide product, overcoming the limitations of direct bacterial collagenase treatment.
2Adaptability or versatility
If multiple proteolytic enzymes are used in combination to produce desired peptides, then peptide diversity increases, but the number of reaction steps and production complexity increase
Solution Approach 1:
The invention makes the single plant-derived protease (zingibain) perform multiple functions: it degrades collagen, produces diverse tripeptides including X-Hyp-Gly, and generates other bioactive peptides. This multi-functionality eliminates the need for multiple specialized enzymes while maintaining peptide diversity.
Solution Approach 2:
The invention merges the functions of multiple proteolytic enzymes into a single plant-derived protease system. Instead of using separate enzymes for different cleavage sites, one enzyme (zingibain) performs all necessary degradations to produce the desired peptide profile from collagen.
3Manufacturing precision
If artificial synthesis or multi-step enzymatic treatment is used to produce X-Hyp-Gly peptides, then desired tripeptides can be obtained, but production cost and process time increase
Solution Approach 1:
The invention performs preliminary selection of an appropriate enzyme (plant-derived protease with specific cleavage characteristics) before the degradation process. This preliminary action ensures that the single-step treatment produces the desired X-Hyp-Gly tripeptides directly, avoiding the need for subsequent purification or additional enzymatic steps.
Solution Approach 2:
The invention skips the intermediate steps of artificial synthesis or multi-step enzymatic treatment by using a single plant-derived protease that directly converts collagen to X-Hyp-Gly tripeptides in one reaction step, significantly accelerating the production process.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method efficiently produces collagen peptide compositions with high DPP-4 inhibitory activity, effectively inhibiting glucose level elevation when orally administered, reducing the complexity and cost of production.
Implementation Method 1
there are a large number of proteolytic enzymes that hydrolyze the collagens or gelatin; and studies have been conducted to efficiently generate biologically-active peptides by any of those enzymes
Implementation Method 2
degrading a collagen and/or gelatin with the addition of a ginger rhizome-derived enzyme into the collagen and/or gelatin solution to generate the peptide
Data Source
AI summary
Provided are methods of producing a novel collagen peptide composition, and a DPP-4 inhibitor and antihyperglycemics that comprise the above-mentioned collagen peptide composition. Ginger rhizome-derived enzymes are added to and break down a collagen and/or gelatin solution to generate peptide compositions comprising peptides represented by X-Hyp-Gly (wherein X represents an amino acid residue other than Gly, Hyp, and Pro). The thus obtained collagen peptide composition has a high DPP-4 inhibitory activity and an excellent antihyperglycemic effect.