Ginseng Exosome Purification Using TFF for Cosmetic-Grade Isolation
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Solution Overview
Problem
Existing methods for extracting plant exosomes, such as density gradient ultracentrifugation and filtration, are inefficient for commercial production, leading to impurities and low purity, especially for ginseng exosomes, which are needed for cosmetic applications like skin regeneration and whitening.
Innovation Solution
A method involving centrifugation and tangential-flow filtration (TFF) is used to isolate high-purity ginseng exosomes, ensuring a concentration of 1×10^7 to 1×10^14 per unit volume, maintaining their stability and biological activity for cosmetic use.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Manufacturing precision
If density gradient ultracentrifugation using sucrose gradient is used to extract plant exosomes, then exosomes can be isolated, but the method is difficult to use for commercial production, sucrose mixes into the extract, and high-purity exosome composition cannot be obtained
Solution Approach 1:
The patent extracts and removes sucrose and other impurities from the exosome extraction process. By using a simplified extraction method that eliminates the need for sucrose gradient ultracentrifugation, the invention achieves high-purity exosome composition without the contamination issues of conventional methods, making the process suitable for commercial production.
Solution Approach 2:
The patent changes the extraction parameters by abandoning the density gradient ultracentrifugation approach and adopting a method that uses specific concentration ranges and filtration techniques. This parameter change enables both high purity (10^7 or more exosomes per unit volume) and commercial feasibility by simplifying the production process.
2Manufacturing precision
If filtration method is used to isolate extracellular vesicles, then exosomes can be separated by size, but filtration efficiency is dramatically reduced when extracting from large amounts of plant juice due to adsorption and accumulation in filter pores
Solution Approach 1:
The patent introduces an intermediary substance or medium that facilitates exosome isolation without direct filtration through porous membranes. By using this intermediary approach, the invention avoids the pore clogging and adsorption problems that occur with conventional filtration when processing large volumes of plant juice, maintaining both purity and extraction efficiency.
Solution Approach 2:
The patent replaces the mechanical filtration system with an alternative separation method that does not rely on physical pores. This substitution eliminates the limitation of filter pore capacity and adsorption saturation, enabling efficient extraction from large amounts of plant juice while maintaining high exosome purity.
3Quantity of substance
If conventional extraction methods are used, then exosomes can be obtained, but impurities are mixed in and high concentration (10^7 or more per unit volume) cannot be achieved
Solution Approach 1:
The patent segments the extraction process into distinct stages that separately address concentration and purity. By dividing the extraction into specific steps with defined parameters, the invention achieves both high concentration (10^7 or more exosomes per unit volume) and high purity by systematically separating exosomes from impurities at different stages.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The high-purity ginseng exosomes, rich in ginsenosides Rg1 and Rb1, effectively promote collagen production, hyaluronic acid synthesis, and skin whitening by stimulating anti-oxidant signaling, making them suitable for various cosmetic formulations.
Implementation Method 1
A method involving centrifugation and tangential-flow filtration (TFF) is used to isolate high-purity ginseng exosomes
Implementation Method 2
A method involving centrifugation and tangential-flow filtration (TFF) is used to isolate high-purity ginseng exosomes
Data Source
AI summary
The present invention relates to a functional cosmetic composition comprising plant exosomes derived from ginseng as an active ingredient and a functional cosmetic containing the same. Ginseng exosomes have a bilipid layer structure and are rich in ginsenosides Rb1 and Rg1, which influence skin improvement and whitening. It has been proven that these powerful functional materials, ginsenosides Rb1 and Rg1, are absorbed in large quantities through ginseng exosomes into skin cells, enhance the synthesis of collagen and hyaluronic acid in skin cells, and have the efficacy of inhibiting melanin synthesis. Therefore, the ginseng exosomes rich in ginsenosides Rb1 and Rg1, presented in the present invention, can be beneficially used in functional cosmetic compositions for skin aging prevention, skin whitening, wrinkle improvement and skin cell regeneration.