Low Auto-Fluorescence Glass Substrates for Fluorescent Detection
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Solution Overview
Problem
Conventional fluorescent-detection methods face challenges due to the high cost of silica substrates and the issue of auto-fluorescence from cheaper alternatives like plastics and polymers, which degrade signal quality by introducing background noise.
Innovation Solution
A glass substrate with a specific composition, including between 65.97 mol% to 78.17 mol% SiO2, 2.94 mol% to 12.12 mol% Al2O3, and other oxides, offering high transmittance and low auto-fluorescence, is used to improve the signal-to-noise ratio in fluorescent-detection methods.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If silica substrate is used, then optical quality and low auto-fluorescence are achieved, but cost increases
Solution Approach 1:
The patent modifies the chemical composition parameters of glass substrates by precisely controlling the proportions of SiO2 (65-78 mol%), Al2O3 (2-12 mol%), B2O3 (0-12 mol%), and other oxides to achieve optimal optical properties and minimize auto-fluorescence while maintaining cost-effectiveness
Solution Approach 2:
The patent creates a composite glass material system combining multiple oxide components (SiO2, Al2O3, B2O3, Na2O, K2O, CaO, MgO, ZnO, SrO, BaO, SnO2) in specific ratios to achieve superior optical performance that balances both quality and manufacturing cost
2Ease of manufacture
If plastic or polymer material is used, then cost decreases and ease of manufacture improves, but auto-fluorescence increases causing background noise
Solution Approach 1:
The patent adjusts the glass composition parameters, particularly increasing SiO2 content to 65-78 mol% and optimizing Al2O3 (2-12 mol%) and B2O3 (0-12 mol%) ratios, to fundamentally reduce the glass matrix's auto-fluorescence properties while maintaining manufacturing feasibility
Solution Approach 2:
The patent applies specific compositional modifications to different regions of the glass substrate structure, ensuring that the glass composition specifically targets reduction of auto-fluorescence in the optical path regions while maintaining overall structural integrity and manufacturability
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The glass substrate provides high optical quality, low auto-fluorescence, and improved accuracy in fluorescent-detection methods by minimizing background noise and enhancing signal clarity.
Implementation Method 1
high transmittance and low auto-fluorescence
Implementation Method 2
The term 'auto-fluorescence' as used herein describes the natural or inherent fluorescence produced by substrates for use in fluorescent-detection methods
Data Source
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AI summary
A substrate for use in fluorescent-detection methods is provided. The substrate includes at least one glass substrate portion, the at least one glass substrate portion including: between about 60 mol % to about 80 mol% SiO2; between about 0 mol% to about 15 mol% A12O3; between about 0 mol% to about 15 mol% B2O3; and about 2 mol% to about 50 mol% RxO, wherein R is any one or more of Li, Na, K, Rb, Cs and x is 2, or wherein R is any one or more of Zn, Mg, Ca, Sr or Ba and x is 1.