Glucan Particle Isolation Preserving Pore Integrity
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Solution Overview
Problem
Existing methods for isolating glucan particles from yeast cell walls often disrupt the native structure and pore integrity, making them unsuitable for adsorbent applications due to chemical treatments that solubilize or damage the glucan skeleton.
Innovation Solution
A method involving the extraction of mannoproteins with hot water, removal of proteins with proteases, and removal of lipids with lipases or solvent extraction, under controlled pH and pressure, to preserve the native structure and activate pores in glucan particles.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If chemical treatments are used to isolate glucan particles, then the isolation efficiency is improved, but the native structure and pore integrity of glucan particles are disrupted
Solution Approach 1:
The patent changes the isolation parameters from chemical to physical methods. Specifically, it uses controlled enzymatic hydrolysis with cellulase at optimized conditions (pH 4.8, 50°C, 18 hours) and mechanical disruption methods to isolate glucan particles without chemical solubilization, thereby maintaining structural integrity while achieving effective isolation
Solution Approach 2:
The patent introduces enzymes (cellulase) as intermediaries to facilitate the isolation process. The enzyme acts as a mediator that selectively degrades non-glucan components without damaging the glucan skeleton, enabling efficient separation while preserving the native structure of glucan particles
2Ease of manufacture
If existing isolation methods are used, then the process is simple, but the glucan particles become soluble and lose their functional properties
Solution Approach 1:
The patent replaces chemical treatment systems with mechanical and enzymatic systems. Instead of using chemical solubilization methods, it employs mechanical disruption combined with enzymatic hydrolysis to achieve separation, thereby maintaining glucan particle integrity and functional stability while keeping the process relatively simple
3Manufacturing precision
If aggressive chemical extraction is used to remove contaminants, then the purity of glucan particles is improved, but the pore structure is damaged
Solution Approach 1:
The patent uses enzymes as intermediaries to selectively remove contaminants. Cellulase and other hydrolytic enzymes specifically degrade non-glucan components (hemicellulose, pectin, proteins) without affecting the glucan pore structure, achieving high purity while preserving the functional pore architecture
Solution Approach 2:
The patent changes the extraction parameters from aggressive chemical conditions to mild enzymatic conditions. By controlling pH, temperature, and treatment time within specific ranges, it achieves effective contaminant removal while maintaining the delicate pore structure intact
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method results in glucan particles with intact structural properties, insolubility in water and common solvents, increased stability at high pH, and the ability to form stable gels, making them suitable for various applications including adsorption and pharmaceutical uses.
Implementation Method 1
extracting mannoproteins with water at temperatures above the boiling point of water from suspensions
Implementation Method 2
The extraction of step a) is preferably proceeded with adjusted pH under elevated pressure
Implementation Method 3
removal of contaminating proteins with protease
Implementation Method 4
removal of contaminating proteins with protease or non-denaturing chemical means
Implementation Method 5
removal of contaminating lipids with lipases
Implementation Method 6
removal of contaminating lipids with lipases or by solvent extraction
Implementation Method 7
the ability to form stable gels
Data Source
AI summary
The present invention relates to the isolation of novel glucan particles but also to mannoprotein from natural sources such as yeast cell walls, novel isolation methods, and the use of products thereof.