Gly m 8 Antibody Detection for Low Cross-Reactivity Soy Allergen Testing
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Solution Overview
Problem
Current ELISA assays for detecting soy allergens, particularly Gly m 5 and Gly m 6, face challenges in distinguishing between different bean species and exhibit high cross-reactivity, leading to inaccurate quantification and underestimation of soy content in food samples, especially with complex and highly processed ingredients.
Innovation Solution
Development of a method to produce antibodies that specifically bind to soy Gly m 8 protein by expressing it in plants, preferably tobacco, purifying it, and obtaining antibodies from animals, which are then used to detect soy material in food and feedstuff preparations.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If current ELISA assays use antibodies against Gly m 5 or Gly m 6, then detection capability is provided, but cross-reactivity with other bean species occurs leading to inaccurate quantification
Solution Approach 1:
The patent extracts and isolates a specific soybean protein marker (Gly m 8, 2S albumin) from complex soybean extracts to serve as a unique detection target. This specific protein extraction enables the development of antibodies that bind only to soybean Gly m 8, eliminating cross-reactivity with other bean species while maintaining detection capability
Solution Approach 2:
The patent applies local quality by selecting a specific protein fraction (2S albumin Gly m 8) with unique properties within the soybean protein complex. This localized targeting of a specific protein type with distinct thermal stability and solubility characteristics allows for species-specific detection without affecting other bean proteins
2Productivity
If antibodies against Gly m 5 are used in sandwich ELISA, then detection is achieved, but underestimation of true Gly m 5 content occurs due to variation in native and denatured fractions
Solution Approach 1:
The patent employs a stable, heat-resistant protein marker (Gly m 8) that maintains its structural integrity and antigenic properties throughout the extraction and detection process. This disposable-like stability of the marker protein ensures consistent antibody binding and accurate quantification without requiring complex fractionation of native and denatured forms
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The antibodies provide accurate and reliable detection of soy material, reducing cross-reactivity with other bean proteins and ensuring consistent quantification, even in complex food preparations, by utilizing the thermal stability and solubility of Gly m 8 protein.
Implementation Method 1
expressing soy Gly m8 protein in plants and, preferably, tobacco plants
Implementation Method 2
purifying the soy Gly m8 protein from said plants and, preferably, tobacco plants
Implementation Method 3
obtaining an antibody from the animal which specifically bind to soy Gly m8 protein
Data Source
AI summary
The present invention relates to the field of antibodies and their application in food and feedstuff quality control. In particular, the invention relates to a method for the manufacture of an antibody that specifically binds to soy Gly m 8 protein wherein said method comprises immunising an animal with the purified soy Glym8 protein, wherein said soy Glym8 protein has been obtained by expressing soy Glym8 protein in plants, and preferably, tobacco plants and purifying the soy Glym8 protein from said plants and, preferably, tobacco plants and obtaining an antibody from the animal which specifically bind to soy Gly m8 protein, wherein the animal will be sacrificed. Moreover, the invention contemplates an antibody obtained by said method as well as the use of said antibody for detecting soy material in a food preparation or feedstuff preparation. Further, a method for detecting soy material in a food preparation or feedstuff preparation and a kit for carrying out said method are provided.