Glycoside Extraction from Plant Material Using Enzymatic Segmentation
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Solution Overview
Problem
Existing methods for extracting and stabilizing glycosides of mono- or diacylglycerol compounds from plant materials, such as rose hips, are not efficient in producing high-content, stable, and safe anti-inflammatory products for commercial use.
Innovation Solution
A method involving milling the plant material, followed by sequential aqueous extractions with temperature and pH control, and the use of cell wall degrading enzymes to isolate glycosides, which are then separated and processed to produce juice, tincture, and solid concentrate products with enhanced anti-inflammatory and antioxidant activities.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If traditional extraction methods are used to obtain glycosides from plant materials, then the extraction process is simple, but the yield of active ingredients is low and the products lack stability
Solution Approach 1:
The extraction process is divided into multiple sequential steps: initial aqueous extraction, pH adjustment to specific levels (pH 2-4), filtration, and concentration. This segmentation allows each step to optimize for specific objectives - initial extraction for yield, pH adjustment for stability, and filtration for purity - thereby increasing overall glycoside yield while maintaining manageable process complexity through systematic breakdown of the extraction workflow
Solution Approach 2:
The method employs controlled pH changes (adjusting to pH 2-4) and temperature control during extraction and concentration steps. These parameter changes optimize the solubility and stability of glycoside compounds, preventing degradation and maximizing yield. The pH adjustment specifically targets the stability window for glycoside compounds, while temperature control during concentration preserves active ingredient integrity
2Quantity of substance
If high concentration of active ingredients is achieved, then product efficacy is improved, but product stability and safety are compromised
Solution Approach 1:
The method controls pH within the specific range of pH 2-4 during extraction and concentration steps. This pH control is critical as it maintains the stability of glycoside compounds, preventing degradation that would occur at higher or lower pH levels. The controlled acidic environment preserves the integrity of active ingredients while achieving high concentration, thereby maintaining both efficacy and stability
Solution Approach 2:
The method uses controlled pH adjustment as an intermediary mechanism to bridge the gap between high concentration achievement and product stability. By introducing pH control as an intermediate step between extraction and final product formulation, the process ensures that high concentrations of glycosides are obtained while maintaining stability through the buffered acidic environment
3Productivity
If extraction efficiency is increased, then production time is reduced, but product quality and purity are compromised
Solution Approach 1:
The extraction process is segmented into distinct functional steps: initial aqueous extraction for efficiency, pH adjustment for quality control, filtration for purity, and concentration for final formulation. Each segment performs a specific function optimally - the initial extraction maximizes yield while subsequent controlled steps ensure quality and purity, achieving both high productivity and manufacturing precision through systematic division of labor
Solution Approach 2:
The method maintains continuous controlled acidic conditions (pH 2-4) throughout the extraction and concentration process. This continuous pH control ensures that the extraction efficiency is maintained while simultaneously preserving product quality and purity by preventing degradation at any stage. The uninterrupted controlled environment allows efficient extraction without compromising the final product characteristics
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method yields products with potent anti-inflammatory and antioxidant properties, effectively treating inflammatory conditions like arthritis, while ensuring the stability and safety of the active ingredients.
Implementation Method 1
The method comprises the steps of: (i) optionally milling the plant material, (ii) extracting the optionally milled plant material with a first aqueous extraction solution obtaining a first liquid phase and a first solid phase
Implementation Method 2
extracting the optionally milled plant material with a first aqueous extraction solution obtaining a first liquid phase and a first solid phase
Implementation Method 3
separating the liquid phase from the solid phase to obtain a glycoside of mono- or diacylglycerol product
Data Source
AI summary
The present invention provides a method of preparing a glycoside of a mono- or diacylglycerol product from a plant material. The method comprises the steps of: (i) optionally milling the plant material, (ii) extracting the optionally milled plant material with a first aqueous extraction solution obtaining a first liquid phase and a first solid phase, (iii) separating the liquid phase from the solid phase to obtain a glycoside of mono- or diacylglycerol product, (iv) extracting the solid phase from step (iii) with a second extraction solution obtaining a second liquid phase and a second phase, and (v) separating the second liquid phase from the second solid phase to obtain a second and a third glycoside of mono- or diacylglycerol product, wherein the second extraction solution further comprises a cell wall degrading enzyme or a mixture of cell wall degrading enzymes.