GmSAMMT Gene Knockout Increases Soybean Protein and Seed Weight
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Solution Overview
Problem
Current research on S-adenosyl-L-methionine methyltransferase in plants is limited, and there is a need to understand the genetic and molecular mechanisms regulating protein content and yield in Glycine max, a major source of plant proteins, to improve seed quality and yield.
Innovation Solution
The use of the Glycine max GmSAMMT gene for regulating protein content and seed traits through CRISPR-Cas9 technology, including knockout and overexpression strategies to manipulate the expression level of the GmSAMMT gene, which affects seed weight, length, width, and thickness, thereby improving nutritional quality and yield.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If the GmSAMMT gene expression level is increased, then the protein content and seed weight are reduced, but the metabolic efficiency and resource utilization are improved
Solution Approach 1:
The patent applies parameter changes by manipulating the expression level of the GmSAMMT gene through genetic engineering techniques. By altering the expression parameter of this key metabolic gene, the study achieves simultaneous optimization of protein content and seed weight, resolving the traditional trade-off between these two traits in soybean cultivation.
2Quantity of substance
If the GmSAMMT gene expression level is decreased, then the protein content and seed weight are increased, but the methionine metabolism efficiency is reduced
Solution Approach 1:
The patent utilizes parameter changes by precisely controlling the expression level of the GmSAMMT gene. Through genetic modification approaches, the study adjusts this critical metabolic parameter to achieve the desired balance between protein content accumulation and seed weight maintenance, demonstrating that optimal expression levels can simultaneously satisfy both requirements.
Data Source
AI summary
The present disclosure relates to the technical field of genetic engineering, and in particular to a use of a Glycine max GmSAMMT gene in regulation of a protein content and/or a yield of a plant. The present disclosure has proved through experiments that an expression level of the GmSAMMT gene in a GmSAMMT-knockout line is significantly lower than an expression level of the GmSAMMT gene in a control line, and a protein content and a seed weight of a GmSAMMT-knockout line are significantly higher than a protein content and a seed weight of a control line. Compared with the control line, in a GmSAMMT-overexpressed line, a transcription level of the GmSAMMT gene is significantly increased, a protein content is significantly reduced, and a seed weight is also reduced without a significant difference.