Goat Colostrum Freeze-Dried Powder With Nested Probiotic Encapsulation
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Solution Overview
Problem
Existing probiotic products face challenges in maintaining high activity and sufficient quantity to colonize the human intestine due to inactivation during processing, transportation, storage, and digestion.
Innovation Solution
A high-bioactivity goat colostrum composite freeze-dried powder is prepared using modified phytosterol, probiotic liposomes encapsulated with soybean phosphatidylcholine and modified chitosan, and mannose oligosaccharides to protect probiotics, enhancing their survival and colonization in the intestine.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If probiotics are used in conventional forms, then they can be easily manufactured and administered, but they suffer from inactivation during processing, transportation, storage, and digestion, resulting in insufficient colonization of the human intestine
Solution Approach 1:
The patent employs a multi-layer nested encapsulation structure where probiotics are first encapsulated within liposomes (inner layer), then these liposomes are further encapsulated within chitosan nanoparticles (outer layer), forming a protective core-shell structure. This nested design provides progressive protection against environmental stresses and digestive enzymes, significantly improving probiotic survival rate while maintaining manageable complexity through systematic layering.
Solution Approach 2:
The invention utilizes composite materials combining two different encapsulation systems: liposomes (phospholipid-based vesicles) and chitosan nanoparticles (polysaccharide-based particles). This composite approach leverages the complementary properties of both materials - liposomes provide membrane-like protection and chitosan offers enzymatic resistance and mucoadhesion - creating a synergistic protective system that enhances probiotic reliability.
2Reliability
If probiotics are protected through multiple encapsulation layers, then their survival rate improves, but the manufacturing process becomes more complex
Solution Approach 1:
The manufacturing process employs preliminary action by first preparing and characterizing the liposome encapsulation system separately, then preparing the chitosan nanoparticles separately, and finally combining them. This stepwise preliminary preparation allows for optimization of each encapsulation layer independently before integration, reducing the complexity of the overall manufacturing process while maintaining high probiotic activity.
Solution Approach 2:
The complex encapsulation process is segmented into distinct modular steps: (1) liposome formation with probiotics, (2) chitosan nanoparticle preparation, and (3) assembly of the core-shell structure. This segmentation allows each module to be independently optimized and controlled, making the overall complex process more manageable and easier to manufacture at scale.
3Quantity of substance
If probiotics are encapsulated in liposomes and chitosan nanoparticles, then their colonization number in the intestine increases, but the production cost and process time increase
Solution Approach 1:
The invention optimizes key parameters such as liposome size (50-200 nm), chitosan nanoparticle size (20-50 nm), pH conditions, and temperature to achieve rapid encapsulation and stabilization. By carefully controlling these parameters, the process achieves high colonization numbers through enhanced intestinal adhesion and protection, while minimizing production time through optimized reaction conditions and streamlined processing steps.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution improves the survival rate and colonization number of probiotics in the intestine, maintaining their probiotic effects by reducing inactivation and enhancing stability during processing, storage, and transportation.
Implementation Method 1
prepare probiotic liposomes by encapsulating probiotics with the modified phytosterol and soybean phosphatidylcholine
Implementation Method 2
prepare composite probiotic liposome freeze-dried powder by encapsulating probiotic liposomes with the modified chitosan
Implementation Method 3
composite probiotic liposome freeze-dried powder
Data Source
AI summary
A high-bioactivity goat colostrum composite freeze-dried powder relates to the technical field of food, comprising the following components in parts by weight: 60-70 parts of goat colostrum powder, 5-8 parts of composite probiotic liposome freeze-dried powder, and 6-9 parts of mannose oligosaccharides; the composite probiotic liposome freeze-dried powder is prepared from the following components: gallic acid, phytosterol, soybean phosphatidylcholine, modified chitosan, and probiotic powder; the probiotic powder is Bifidobacterium longum powder or Lactobacillus acidophilus powder. The invention proposes to prepare modified phytosterol by esterification reaction of gallic acid and phytosterol, and to prepare probiotic liposomes by encapsulating probiotics with the modified phytosterol and soybean phosphatidylcholine, and at the same time introduce carboxymethyl into chitosan to prepare modified chitosan, and to prepare composite probiotic liposome freeze-dried powder by encapsulating probiotic liposomes with the modified chitosan, thereby protecting probiotics, thereby reducing the inactivation of probiotics, and improving their survival rate.