Gold-Binding Peptide Sequence SQMMGHMGHGNMNHMNHGGKFDFHH

Solution Overview

Problem

Current peptides are not effective in binding to gold, lacking a specific amino acid sequence that enables strong recognition and binding.

Innovation Solution

A peptide with the amino acid sequence SQMMGHMGHGNMNHMNHGGKFDFHH, derived from the 382nd to 406th amino acids of CueO, is used, which is chemically modified with biotin-polyethylene glycol to prevent non-specific interactions, allowing it to bind effectively to gold nanoparticles.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If conventional peptides are used, then the structure is simple, but the binding ability to gold is insufficient

Engineering Contradiction:

Improvebinding abilityVSAvoidpeptide structure complexity

Core Design Contradiction:

ReliabilityVSDevice complexity

Solution Approach 1:

The patent applies parameter changes by modifying the amino acid sequence parameters of the peptide. Specifically, it identifies and utilizes a particular sequence (SEQ ID NO: 01) with specific compositional parameters that enable gold binding, transforming a conventional non-binding peptide into a gold-binding peptide through parameter optimization of its amino acid composition and arrangement.

Inventive Principle:

Principle #35Parameter changes

2Reliability

If a specific amino acid sequence is introduced for gold binding, then the binding specificity is improved, but the ease of manufacture decreases

Engineering Contradiction:

Improvebinding specificityVSAvoidpeptide synthesis difficulty

Core Design Contradiction:

ReliabilityVSEase of manufacture

Solution Approach 1:

The patent applies the extraction principle by isolating and utilizing only the essential gold-binding amino acid sequence from the full CueO protein. By extracting and synthesizing only the critical 25-amino acid segment (SEQ ID NO: 01) responsible for gold binding, the invention simplifies manufacturing while maintaining binding specificity, avoiding the need to produce the entire parent protein.

Inventive Principle:

Principle #2Taking out (Extraction)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The peptide successfully binds to gold nanoparticles, forming aggregates and demonstrating strong recognition, as evidenced by color change and electrophoresis results, indicating its capability to form stable complexes with gold.

Implementation Method 1

A peptide with the amino acid sequence SQMMGHMGHGNMNHMNHGGKFDFHH, derived from the 382nd to 406th amino acids of CueO, is used, which is chemically modified with biotin-polyethylene glycol to prevent non-specific interactions, allowing it to bind effectively to gold nanoparticles.

Methodology Applied

Scientific EffectSpecific recognition and binding:

Implementation Method 2

The peptide successfully binds to gold nanoparticles, forming aggregates and demonstrating strong recognition, as evidenced by color change and electrophoresis results, indicating its capability to form stable complexes with gold.

Methodology Applied

Scientific EffectAggregation:

Data Source

PatentUS8674068B2Peptide capable for binding to gold

Publication Date: 2014.03.18 PANASONIC HOLDINGS CORP

AI summary

The present invention provides a novel peptide capable for binding to gold. The peptide consists of an amino acid sequence represented by SQMMGHMGHGNMNHMNHGGKFDFHH (SEQ ID NO: 01).