GPER Recombinant Protein Expression Stability

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Solution Overview

Problem

Current methods for studying and developing GPER receptor agonists face challenges in efficiently producing and purifying recombinant GPER proteins, which are crucial for understanding the receptor's signaling mechanisms and developing effective therapeutic agents.

Innovation Solution

The development of a recombinant protein of GPER, encoded by a specific synthetic DNA sequence, which is expressed and purified using a recombinant host and affinity chromatography, enabling the production of stable monomers and dimers for use in drug screening and other applications.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If traditional methods are used for producing and purifying recombinant GPER proteins, then the production process is simple, but the expression stability and purification efficiency are insufficient

Engineering Contradiction:

Improveexpression stabilityVSAvoidproduction process complexity

Core Design Contradiction:

ReliabilityVSDevice complexity

Solution Approach 1:

The patent introduces a tag sequence as an intermediary element that facilitates both expression stabilization and purification. The tag sequence acts as a mediator between the GPER protein and the purification system, enabling efficient isolation while maintaining expression stability through optimized codon usage and structural elements

Inventive Principle:

Principle #24Intermediary (Mediator)

2Productivity

If traditional purification methods are used, then the process is straightforward, but the purification efficiency and protein quality are insufficient

Engineering Contradiction:

Improvepurification efficiencyVSAvoidpurification process complexity

Core Design Contradiction:

ProductivityVSDevice complexity

Solution Approach 1:

The tag sequence serves as a purification intermediary that enables high-efficiency isolation of GPER proteins through specific binding interactions. This mediator approach allows rapid purification with high recovery rates while maintaining protein functionality, overcoming the limitations of traditional purification methods

Inventive Principle:

Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent optimizes various parameters including codon usage, tag sequence composition, and expression conditions to enhance purification efficiency. By changing these parameters, the system achieves higher protein yield and purity while maintaining process feasibility

Inventive Principle:

Principle #35Parameter changes

3Reliability

If existing recombinant protein methods are used, then the production cost is low, but the protein stability and functionality for drug screening are insufficient

Engineering Contradiction:

Improveprotein stabilityVSAvoidmanufacturing ease

Core Design Contradiction:

ReliabilityVSEase of manufacture

Solution Approach 1:

The patent employs codon optimization and structural parameter adjustments to enhance protein stability without significantly increasing manufacturing complexity. These parameter changes ensure the recombinant GPER proteins maintain proper folding and functionality essential for drug screening applications

Inventive Principle:

Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

This approach allows for the stable expression and purification of GPER recombinant proteins, facilitating the study of GPER signaling and the development of GPER agonists, thereby addressing the limitations of existing methods.

Implementation Method 1

The development of a recombinant protein of GPER, encoded by a specific synthetic DNA sequence, which is expressed and purified using a recombinant host

Methodology Applied

Scientific EffectGenetic expression: