Concentration Gradient Fluorescence Calibration Sheet for Laser Microarray Scanner
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Solution Overview
Problem
Current fluorescence calibration sheets for microarray chip scanners are complex and costly to produce, making them unsuitable for widespread use in biochip technology, where precise calibration is crucial for accurate detection and measurement.
Innovation Solution
A concentration gradient fluorescence calibration sheet featuring an inorganic nano fluorescent liquid lattice on a glass substrate, with uniformly distributed sub-lattices and controlled solution concentrations, allowing for easy preparation and calibration of laser microarray chip scanners.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If inorganic fluorescent materials are used to prepare calibration sheets, then fluorescence emission stability and repeatability are improved, but preparation complexity and cost increase
Solution Approach 1:
The patent uses phosphor dots as a simplified copy or representation of fluorescent materials. These phosphor dots are deposited on glass substrates to create calibration sheets that replicate the essential calibration function without requiring complex inorganic fluorescent material preparation, thus reducing complexity while maintaining reliability
Solution Approach 2:
The patent employs phosphor dots which can be easily deposited and replaced, serving as a cost-effective alternative to permanent inorganic fluorescent materials. The calibration sheets can be prepared quickly and discarded after use, eliminating the need for complex, expensive, and long-lasting fluorescent material preparation
2Measurement precision
If conventional fluorescence calibration sheets are used, then calibration accuracy is maintained, but production cost and preparation time increase
Solution Approach 1:
The patent changes the material parameter from inorganic fluorescent materials to phosphor dots, and adjusts the preparation method to a simpler deposition process. This parameter change maintains calibration accuracy while significantly improving production efficiency and reducing costs
Solution Approach 2:
The calibration sheet is segmented into multiple phosphor dot arrays with different concentrations arranged in a grid pattern. This segmentation allows for easy preparation and quick scanning, improving productivity while maintaining the precision needed for accurate calibration across different concentration levels
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The calibration sheet provides accurate and repeatable calibration results, ensuring scanner performance with a high correlation coefficient, thus addressing the complexity and cost issues of existing methods while enhancing operational efficiency and applicability.
Implementation Method 1
an inorganic nano fluorescent liquid lattice disposed on a surface of the glass substrate
Implementation Method 2
inorganic fluorescent mixed liquid droplets of each inorganic nano fluorescent liquid sub-lattice
Data Source
AI summary
A concentration gradient fluorescence calibration sheet has a glass substrate and an inorganic nano fluorescent liquid lattice disposed on a surface of the glass substrate. The inorganic nano fluorescent liquid lattice has a plurality of inorganic nano fluorescent liquid sub-lattices. Inorganic fluorescent mixed liquid droplets of each inorganic nano fluorescent liquid sub-lattice are arranged in A rows×B columns. The solution concentration of the same row of inorganic nano fluorescent liquid droplets in the inorganic nano fluorescent liquid sub-lattices is the same, and the solution concentrations of two adjacent rows of inorganic nano fluorescent liquid droplets change in multiples. The concentration gradient fluorescence calibration sheet for the laser microarray chip scanner is utilized to calibrate a scanner under test and a first row of inorganic nano fluorescent liquid droplets in the inorganic nano fluorescent liquid sub-lattices are taken as index marks.