Gradient Pore Non-Woven Substrate for Platelet Separation

Solution Overview

Problem

Current separation substrates for platelets have a high megakaryocyte blocking rate but a low platelet permeation rate, making them inefficient for producing platelets from megakaryocytes, especially with the challenges of declining blood donor populations and the need for alternative platelet sources.

Innovation Solution

A separation substrate made of non-woven fabric with an average pore diameter of 2.0 μm to 15.0 μm and a thickness of 10 μm to 500 μm, combined with specific resin materials and air permeability, is used to achieve a high megakaryocyte blocking rate and platelet permeation rate, enhancing the separation performance.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If a separation substrate with small pore diameter is used to block megakaryocytes, then the megakaryocyte blocking rate is improved, but the platelet permeation rate deteriorates

Engineering Contradiction:

Improvemegakaryocyte blocking rateVSAvoidplatelet permeation rate

Core Design Contradiction:

ReliabilityVSProductivity

Solution Approach 1:

The patent applies local quality by creating a gradient structure where the pore diameter varies through the thickness of the non-woven fabric. The pore diameter is smaller at the upstream side (closer to 2.0 μm) to effectively block megakaryocytes, and gradually increases toward the downstream side (up to 15.0 μm) to facilitate platelet permeation. This spatial variation in pore size allows each region to perform its specific function optimally.

Inventive Principle:

Principle #3Local quality

Solution Approach 2:

The patent utilizes parameter changes by optimizing the average pore diameter within the range of 2.0 μm to 15.0 μm and controlling the thickness within 10 μm to 500 μm. By adjusting these parameters, the substrate achieves both high megakaryocyte blocking and high platelet permeation rates simultaneously, resolving the contradiction between blocking efficiency and permeation efficiency.

Inventive Principle:

Principle #35Parameter changes

2Reliability

If the non-woven fabric thickness is increased to improve separation performance, then the megakaryocyte blocking rate is improved, but the platelet permeation rate deteriorates

Engineering Contradiction:

Improveseparation performanceVSAvoidplatelet permeation rate

Core Design Contradiction:

ReliabilityVSProductivity

Solution Approach 1:

The patent applies local quality by creating a gradient structure where the pore diameter varies through the thickness of the non-woven fabric. The pore diameter is smaller at the upstream side (closer to 2.0 μm) to effectively block megakaryocytes, and gradually increases toward the downstream side (up to 15.0 μm) to facilitate platelet permeation. This spatial variation in pore size allows each region to perform its specific function optimally.

Inventive Principle:

Principle #3Local quality

Solution Approach 2:

The patent utilizes parameter changes by optimizing the average pore diameter within the range of 2.0 μm to 15.0 μm and controlling the thickness within 10 μm to 500 μm. By adjusting these parameters, the substrate achieves both high megakaryocyte blocking and high platelet permeation rates simultaneously, resolving the contradiction between blocking efficiency and permeation efficiency.

Inventive Principle:

Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The proposed substrate configuration significantly improves the megakaryocyte blocking rate and platelet permeation rate, enabling more effective production of platelets, addressing the inefficiencies of existing technologies.

Implementation Method 1

a separation substrate comprising non-woven fabric for separating a platelet from a cell suspension containing a megakaryocyte and the platelet, in which an average pore diameter of the separation substrate is 2.0 μm to 15.0 μm

Methodology Applied

Scientific EffectPhysical filtration: Filter (physical)

Data Source

PatentUS11512275B2Separation substrate, cell separation filter, and method for producing platelet

Publication Date: 2022.11.29 FUJIFILM CORP

AI summary

An object of the present invention is to provide a separation substrate having a high megakaryocyte blocking rate and a high platelet permeation rate, and a cell separation filter and a method for producing a platelet which use the same. The separation substrate of the present invention is a separation substrate including non-woven fabric for separating a platelet from a cell suspension containing a megakaryocyte and the platelet, in which an average pore diameter of the separation substrate is 2.0 μm to 15.0 μm, and a thickness of the separation substrate is 10 μm to 500 μm.