Grapevine Callus Viniferin Production via Stevioside Induction
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Solution Overview
Problem
Current methods for increasing resveratrol content in grapes are limited to post-harvest treatments or single environmental factor manipulations, lacking a method for mass production of viniferin using grape tree cell culture.
Innovation Solution
A method involving transplanting grape tree tissue fragments onto a callus induction medium, followed by growth medium culture and treatment with methyl jasmonate and stevioside for shaking or static culture to induce production of delta-viniferin and epsilon-viniferin.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If post-harvest treatments or single environmental factor manipulations are used to increase resveratrol content, then resveratrol content is improved, but the production method is limited and cannot achieve mass production of viniferin
Solution Approach 1:
The patent applies parameter changes by systematically varying multiple environmental factors (light quality, temperature, nutrient composition) and treatment conditions (inoculation timing, fungal strain selection) to optimize viniferin production. This multi-parameter optimization enables mass production of viniferin while maintaining versatility in production methods, resolving the contradiction between production quantity and method adaptability.
2Quantity of substance
If conventional treatment methods are applied to grapes, then resveratrol content is increased, but viniferin mass production is not achieved
Solution Approach 1:
The patent segments the production process into distinct stages: grape cultivation with controlled environmental factors, fungal inoculation phase, and post-harvest treatment phase. This segmentation allows each stage to be optimized independently for viniferin production while maintaining overall process simplicity, achieving both high viniferin concentration and ease of manufacture.
Solution Approach 2:
The patent applies preliminary action by pre-treating grapes with specific environmental conditions and fungal inoculation before harvest to prime the grapes for viniferin production. This preliminary preparation simplifies the subsequent extraction and processing steps, achieving high viniferin concentration while maintaining production process simplicity.
3Productivity
If single treatment methods are used during grape growing, then production process is simple, but viniferin production efficiency is low
Solution Approach 1:
The patent applies universality by using a multi-functional production system that combines plant cultivation, fungal inoculation, and metabolite induction in a single integrated process. This system serves multiple functions simultaneously (grape growth promotion, viniferin induction, and natural fermentation), achieving high production efficiency while minimizing the need for separate complex equipment and processes.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method efficiently produces viniferin, a stilbene compound with anti-cancer, anti-oxidant, and skin whitening properties, suitable for health foods, cosmetics, and pharmaceutical applications, demonstrating effective mass production from grape tree callus cultures.
Implementation Method 1
Resveratrol and its derivatives play critical roles as phytoalexins and anti-oxidants in plant defense reactions
Implementation Method 2
adding an activity-inducing agent and a solubilizing agent to the grown callus, followed by shaking culture
Data Source
AI summary
The present invention relates to a method for mass-production of viniferin using stevioside from cell culture of grape tree tissue. Viniferin is known to be effective for protection of liver, anticancer, antioxidant, and skin whitening, have an effect of inhibiting oxidation of low-density lipoprotein and high-density lipoprotein and inhibiting the proliferation and migration of vascular smooth muscle cells. Therefore, the present invention is very useful for the mass production of viniferin among the useful substances (stilbene compounds) from a callus derived from the anther tissue of the grape plant, which is very important for the related industries.