Graphene Oxide Aptamer Biosensor for Rapid Phthalate Detection

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Solution Overview

Problem

Current devices for measuring phthalate-based materials are expensive, difficult to access, and struggle with rapid and accurate measurements.

Innovation Solution

An electrochemical aptamer biosensor is developed, comprising an electrode with a composite graphene oxide layer and metal nanoparticles, and target material-specific aptamers, which measures phthalate-based materials through electrochemical signals generated by shape changes in aptamers.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Measurement precision

If chromatographs are used to measure phthalate-based materials, then measurement accuracy is improved, but device cost and accessibility worsen

Engineering Contradiction:

Improvemeasurement accuracyVSAvoiddevice cost

Core Design Contradiction:

Measurement precisionVSDevice complexity

Solution Approach 1:

The patent employs a disposable electrochemical aptamer biosensor that can be manufactured at low cost using simple materials including graphene oxide, metal nanoparticles, and aptamers. The sensor is designed for single-use to maintain accuracy while minimizing cost and complexity concerns

Inventive Principle:

Principle #27Cheap short-living objects (Disposable)

Solution Approach 2:

The patent changes the detection parameter from complex chromatographic separation to simple electrochemical signal measurement. By measuring electrochemical signals that change in response to target material binding, the system achieves high measurement accuracy without requiring expensive chromatograph equipment

Inventive Principle:

Principle #35Parameter changes

2Measurement precision

If chromatographs are used to measure phthalate-based materials, then measurement accuracy is improved, but measurement speed worsens

Engineering Contradiction:

Improvemeasurement accuracyVSAvoidmeasurement speed

Core Design Contradiction:

Measurement precisionVSProductivity

Solution Approach 1:

The patent replaces the mechanical chromatographic separation system with an electrochemical detection system. The aptamer-target binding events are directly transduced into electrochemical signals through the conductive graphene oxide layer, eliminating the need for complex separation processes and enabling rapid measurement while maintaining accuracy

Inventive Principle:

Principle #28Mechanics substitution (Replace mechanical system)

3Device complexity

If conventional sensors are used, then device simplicity is maintained, but measurement accuracy and detection limit worsen

Engineering Contradiction:

Improvedevice simplicityVSAvoiddetection limit

Core Design Contradiction:

Device complexityVSMeasurement precision

Solution Approach 1:

The patent uses a composite material system consisting of graphene oxide plates combined with metal nanoparticles to create a highly conductive and sensitive sensing layer. This composite structure provides both structural simplicity and enhanced measurement accuracy with low detection limits

Inventive Principle:

Principle #40Composite materials

Solution Approach 2:

The patent applies functional materials specifically at the sensor surface where they are most needed. The graphene oxide and metal nanoparticles are localized to the electrode surface to maximize electrochemical signal generation, while the rest of the device remains simple

Inventive Principle:

Principle #3Local quality

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The biosensor allows for convenient, rapid, and accurate measurement of phthalate-based materials with a wide measurable concentration range and low detection limit, enhancing economic feasibility and precision.

Implementation Method 1

the graphene oxide plates may have the sandwich structure by π-π interaction with the ionic liquid

Methodology Applied

Scientific Effectπ-π interaction:

Implementation Method 2

The 5′-end of the target material-specific aptamer may specifically bind to the metal nanoparticles of the composite graphene oxide layer

Methodology Applied

Scientific EffectSpecific binding:

Implementation Method 3

The electrochemical aptamer biosensor may measure a concentration of a target material through an electrochemical signal that is reduced as the target material changes a shape of the target material-specific aptamer having nanoparticles

Methodology Applied

Scientific EffectElectrochemical signal:

Data Source

PatentUS12455257B2Electrochemical aptamer biosensor that detects target material

Publication Date: 2025.10.28 UI (UNIVERSITY IND FOUNDATION) YONSEI UNIVERSITY

AI summary

Provided are an electrochemical aptamer biosensor that detects a target material, which is a phthalate-based material, a method of manufacturing the same, and a method of analyzing the target material using the same, and the electrochemical aptamer biosensor may be simply manufactured and may conveniently and quickly measure the target material, and may have a wide measurable concentration range of the target material and a low detection limit and thus may accurately and precisely measure a concentration of the target material in a sample for analysis.