Graphene Oxide Influenza Chip Using Resistance-Based Virus Detection
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Solution Overview
Problem
Existing influenza virus detection methods suffer from low sensitivity, complex processes, and high costs, particularly in colloidal gold immunochromatography and PCR, which are not effective for low viral loads and require extensive sample preparation.
Innovation Solution
A graphene oxide-based detection chip with integrated electrodes and monoclonal antibodies, utilizing fluorescence energy resonance transfer to detect influenza virus through changes in electrical resistance upon laser irradiation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of operation
If colloidal gold immunochromatography is used for influenza virus detection, then the detection process is simple and rapid, but the detection sensitivity is low and cannot detect low viral loads
Solution Approach 1:
The patent uses graphene oxide as a substrate material that combines electrical conductivity with large surface area for antibody immobilization. The fluorescent labels (quantum dots or carbon dots) provide high brightness and photostability. This composite structure enables both operational simplicity and high detection sensitivity by integrating multiple material advantages into a single detection platform.
Solution Approach 2:
The patent replaces the visual colorimetric detection system of traditional immunochromatography with an electrical resistance measurement system. By measuring changes in electrical resistance of the graphene oxide film when fluorescently-labeled antibodies bind to viral antigens, the system achieves higher sensitivity while maintaining rapid and simple operation without requiring complex optical equipment.
2Measurement precision
If traditional detection methods like ELISA or PCR are used, then the detection sensitivity is improved, but the detection process becomes complicated and time-consuming
Solution Approach 1:
The patent merges the high sensitivity of fluorescent detection with the simplicity of immunochromatography format. By combining fluorescently-labeled monoclonal antibodies with graphene oxide's electrical properties, the system integrates sample application, antibody-antigen binding, and signal detection into a single integrated chip, eliminating the need for separate complex procedures while maintaining high detection sensitivity.
Solution Approach 2:
The patent substitutes complex optical detection systems with a simplified electrical resistance measurement approach. The graphene oxide film's electrical properties change when fluorescently-labeled antibodies bind to viral antigens, allowing detection through simple electrical measurements rather than complex optical instrumentation, thus reducing device complexity while maintaining sensitivity.
3Reliability
If conventional detection methods are used, then the established protocols are available, but the detection time is long and cost is high
Solution Approach 1:
The patent replaces time-consuming optical scanning and analysis with rapid electrical resistance measurements. The graphene oxide film provides immediate electrical signal changes upon antibody-antigen binding, enabling detection within minutes rather than hours, while the established immunochromatography protocol ensures method reliability and ease of standardization.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The chip provides high sensitivity and rapid detection of influenza virus, enabling early identification with simplified procedures and reduced costs.
Implementation Method 1
utilizing fluorescence energy resonance transfer to detect influenza virus through changes in electrical resistance upon laser irradiation
Implementation Method 2
detecting a current across the graphene oxide film by using the first electrode and the second electrode; and determining that the sample solution contains influenza virus in the case where a change occurs in the current across the graphene oxide film
Implementation Method 3
a first electrode and a second electrode, connected to both ends of the graphene oxide film in a second direction perpendicular to the first direction
Implementation Method 4
the fluorescent label includes a C=C-C=C conjugated double bond
Data Source
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Figure 3~4
AI summary
There is provided an influenza virus detection chip and a method for detecting influenza virus therewith. An influenza virus detection chip comprising: a graphene oxide film; a bonding pad disposed on one side of the graphene oxide film in a first direction; and a first electrode and a second electrode, connected to both ends of the graphene oxide film in a second direction perpendicular to the first direction, wherein a first monoclonal antibody with a fluorescent label is included in the bonding pad, and a second monoclonal antibody is included in the graphene oxide film, and wherein the fluorescent label comprises a C=C-C=C conjugated double bond.