Graphene Oxide PNA Probe Kit for Multiplexed Nucleic Acid Detection
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Solution Overview
Problem
Current methods for detecting nucleic acids, such as microRNA, face challenges with multiplexed detection, real-time monitoring, and high costs, particularly in using existing molecular biological and biochemical techniques.
Innovation Solution
A kit and method utilizing graphene oxide and peptide nucleic acid (PNA) probes with fluorescent materials, where the PNA probes are complementary to target nucleic acids and adsorbed on graphene oxide, allowing for the detection of fluorescent light emission upon hybridization, enabling real-time and multiplexed detection of nucleic acids.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If conventional molecular biological or biochemical methods are used for nucleic acid detection, then detection can be performed with established techniques, but multiplexed detection is impossible and real-time detection is difficult
Solution Approach 1:
The invention segments the detection system into distinct functional modules: graphene oxide particles serve as separate detection platforms, each capable of binding different PNA probes with fluorescent labels. This segmentation enables multiple nucleic acid targets to be detected simultaneously in different channels without requiring a single complex integrated system.
Solution Approach 2:
The graphene oxide particles serve as universal platforms that can bind various PNA probes targeting different nucleic acid sequences. The same graphene oxide-based detection mechanism works for multiple targets, making the system multi-functional and enabling multiplexed detection without requiring separate systems for each target.
2Loss of time
If conventional molecular biological methods are used for nucleic acid detection, then detection can be performed, but real-time monitoring is difficult
Solution Approach 1:
The fluorescent labels on PNA probes bound to graphene oxide particles provide continuous signal output throughout the detection process. This continuous fluorescence emission enables real-time monitoring of nucleic acid detection without requiring intermittent sampling or complex time-resolved measurement systems.
3Measurement precision
If conventional nucleic acid detection methods are used, then detection can be performed, but cost of detection is high
Solution Approach 1:
The invention uses inexpensive graphene oxide particles as disposable detection platforms. These particles can be synthesized at low cost and used as single-use reagents in detection assays, eliminating the need for expensive reusable instruments while maintaining detection accuracy through their unique fluorescence quenching properties.
4Adaptability or versatility
If PNA probes with fluorescent materials are used with graphene oxide, then real-time and multiplexed detection is enabled, but the system requires specific material interactions
Solution Approach 1:
The invention creates a composite system combining graphene oxide particles with fluorescently labeled PNA probes. This composite material leverages the unique properties of both components: graphene oxide's fluorescence quenching capability and PNA's high-affinity nucleic acid binding, resulting in a detection system that enables multiplexed and real-time detection through their synergistic interaction.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach allows for the accurate, cost-effective, and real-time detection of nucleic acids, including microRNA, with high sensitivity and specificity, enabling the identification of expression patterns and potential therapeutic substances, and is compatible with high-throughput screening.
Implementation Method 1
graphene oxide (GO), which is an oxidized form of graphene, is capable of quenching a fluorescence signal of organic fluorescent dyes through FRET (Fluorescence Resonance Energy Transfer)
Implementation Method 2
a PNA probe which includes a fluorescent material and is complementary to a target nucleic acid
Implementation Method 3
a strong bond between the graphene oxide and hydrophobic molecules and a single-strand nucleic acid through π-π stacking and/or a hydrogen bond interaction
Implementation Method 4
a strong bond between the graphene oxide and hydrophobic molecules and a single-strand nucleic acid through π-π stacking and/or a hydrogen bond interaction
Data Source
AI summary
The present invention relates a kit for detecting a nucleic acid and a method of detecting a nucleic acid for enabling a multiplexed-detection and real-time detection of a target nucleic acid by using properties of a graphene oxide.