Grass Carp Allergen Markers for Fish Allergy Diagnosis
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Solution Overview
Problem
Current diagnostic methods for fish allergies lack sensitivity and specificity, particularly in detecting allergies induced by a broad spectrum of fish species, leading to inadequate protection and management of symptoms.
Innovation Solution
Identification and utilization of novel allergens such as β-parvalbumin, β-enolase, and aldolase A from grass carp Ctenopharyngodon idella as diagnostic markers, which are used in a method involving polypeptides and nucleic acids to detect IgE antibodies in serum samples for accurate diagnosis of fish allergies.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If currently available commercial diagnostic methods are used to detect fish allergies, then the diagnostic process is simple and quick, but the sensitivity and specificity are insufficient
Solution Approach 1:
The invention segments the fish allergen detection into multiple specific allergen components (parvalbumin, enolase, aldolase) rather than using a single commercial test. This segmentation allows for more precise detection of different fish species-specific allergies, improving both sensitivity and specificity by targeting individual allergenic proteins separately.
Solution Approach 2:
The invention changes the detection parameters by using multiple specific allergen markers (parvalbumin, enolase, aldolase) instead of a single commercial test parameter. This multi-parameter approach enables more accurate differentiation between fish species allergies, thereby improving diagnostic precision without requiring overly complex procedures.
2Adaptability or versatility
If a broad spectrum of fish species allergies is detected, then the diagnostic coverage is improved, but the false positive rate increases
Solution Approach 1:
The invention segments fish allergy detection into species-specific allergen components (parvalbumin, enolase, aldolase from different fish species). This segmentation allows the diagnostic method to cover a broad spectrum of fish species while maintaining high specificity by detecting only the relevant allergens for each species, thereby reducing false positives.
Solution Approach 2:
The invention applies local quality by using different allergen markers for different fish species. Each allergen component is specifically targeted to detect allergies to particular fish species, allowing the diagnostic method to adapt to various fish species while maintaining high precision through species-specific detection.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The use of these allergens significantly improves diagnostic sensitivity and specificity, enabling effective detection of fish allergies and guiding appropriate therapeutic interventions.
Implementation Method 1
contacting a serum or plasma sample taken from the subject with a polypeptide comprising or consisting of any one of SEQ ID NOs: 1-3; detecting in the sample presence of an IgE antibody that specifically binds to the polypeptide
Data Source
AI summary
The present invention provides novel allergens isolated from grass carp Ctenopharyngodon idella, recombinant or modified polypeptides comprising such allergens, nucleic acids encoding the polypeptides as well as related compositions. Also provided are methods and kits for diagnosing fish allergy.