Gravity Electric Field Control Device for Cell Transfection
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Existing methods for increasing cell membrane permeability, such as electroporation, often result in unintended cell destruction, and devices that control gravity are complex and expensive, making it difficult to efficiently transfet genetic material into cells.
Innovation Solution
A device and method that control effective gravity and electric fields to increase cell membrane permeability, using a dual module system to apply zero gravity, microgravity, low gravity, or weighted gravity states to target cells, combined with an electric field to enhance transfection efficiency while minimizing cell damage.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If electroporation is used to increase cell membrane permeability, then transfection efficiency is improved, but cell destruction increases
Solution Approach 1:
The invention changes the physical parameters applied to cells by introducing gravity variation (from 0g to 5g) as a new parameter alongside electric field strength. This allows optimization of transfection conditions by adjusting gravity parameters to reduce cell damage while maintaining transfection efficiency, rather than relying solely on electric field parameters that cause cell destruction.
Solution Approach 2:
The invention introduces dynamic gravity variation (changing from 0g to 5g during the process) rather than static conditions. This dynamic approach allows cells to be in a low-gravity state during electroporation to minimize damage, then transitions to higher gravity for recovery and transfection consolidation, improving overall cell viability.
2Adaptability or versatility
If complex gravity control equipment is used to apply microgravity to cells, then gravity control capability is improved, but device complexity and cost increase
Solution Approach 1:
The invention merges the gravity control function with the existing electroporation device structure. The gravity control unit is integrated into the device housing, and the lifting unit works in conjunction with the electroporation electrodes, combining multiple functions into a unified system rather than requiring separate complex gravity control equipment.
Solution Approach 2:
The invention introduces a lifting unit that acts as an intermediary mechanism to create microgravity conditions. This unit uses simple mechanical lifting of the culture plate to achieve gravity variation, avoiding the need for complex centrifugal or acoustic gravity control systems.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method effectively increases cell membrane permeability, allowing for the efficient transfection of specific substances into a large number of target cells in a short period of time while minimizing cell destruction, thus overcoming the limitations of existing technologies.
Implementation Method 1
the internal module moving independently from the external module within the movement space by an external force transmitted through the external module
Implementation Method 2
a device for applying a zero gravity, microgravity, low gravity, or weighted gravity state
Implementation Method 3
a position detection sensor configured to detect a position of the internal module within the movement space
Implementation Method 4
an actuator configured to move the external module so that the external module has the at least one physical quantity according to the control signal
Implementation Method 5
effective gravity and electric field control device
Data Source
AI summary
The present invention relates to an effective gravity and electric field control device that increases cell membrane permeability by implementing a zero-gravity, microgravity, low-gravity, or weighted gravity state in a module accommodating cells. In addition, the present invention relates to an effective gravity and electric field control device that combines a method for changing gravity and a method for applying an electric field.The effective gravity and electric field control device according to one embodiment of the present invention includes an external module in which a movement space of an internal module is provided and of which at least one physical quantity related to movement is controlled and the internal module moving independently from the external module within the movement space by an external force transmitted through the external module, and the internal module includes an internal space where the target cell and a specific substance to be transfected into the target cell are loaded in a suspension state.