Growth-Factor Bone Graft Composition With Viable Osteogenic Cells
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Existing osteogenic compositions lack enhanced osteoinductive properties and efficacy in bone regeneration, necessitating innovative approaches to improve the effectiveness of biomaterials used in surgical procedures.
Innovation Solution
The development of osteogenic compositions comprising growth-factor augmented cortical bone and cancellous bone, where the cortical bone is demineralized and amplified with growth factors derived from demineralized bone, and the cancellous bone retains viable endogenous osteogenic cells without loosening agents, which are then cryopreserved or lyopreserved.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If traditional demineralized bone matrix is used, then osteoconductive properties are provided, but osteoinductive properties are insufficient
Solution Approach 1:
The patent combines demineralized bone matrix with concentrated growth factors in a single composition, merging osteoconductive and osteoinductive properties into one integrated product that simultaneously provides both structural framework and biological signaling
Solution Approach 2:
The patent pre-concentrates growth factors from demineralized bone into a highly concentrated form during manufacturing, so that when applied to bone defects, the growth factors are already in optimal concentrations without requiring additional processing or activation steps
2Reliability
If growth factors are added to demineralized bone, then osteoinductive properties are enhanced, but manufacturing complexity increases
Solution Approach 1:
The patent uses the demineralized bone itself as the source material for generating growth factors, which then become concentrated within the same matrix structure, creating a self-contained system where the material serves both as carrier and source of active ingredients
Solution Approach 2:
The patent fundamentally changes the concentration parameter of growth factors within the bone matrix, transitioning from trace amounts in traditional DBM to highly concentrated levels through controlled extraction and re-incorporation processes
3Ease of manufacture
If cancellous bone is treated with loosening agents, then cell extraction is facilitated, but cell viability and adhesion are compromised
Solution Approach 1:
The patent eliminates the use of harmful loosening agents entirely, instead using gentle mechanical and enzymatic methods that preserve cell viability while still achieving effective cell extraction and activation from cancellous bone
4Area of stationary object
If demineralized cortical bone is milled and processed, then surface area is increased, but growth factor retention is reduced
Solution Approach 1:
The patent performs growth factor concentration and protection steps during the milling and processing stages, ensuring that growth factors are stabilized and retained within the bone matrix structure before and during mechanical processing
Solution Approach 2:
The patent utilizes the porous structure created by milling and demineralization to trap and retain growth factors within the bone matrix voids, where they are protected from degradation while maintaining increased surface area for biological interaction
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The resulting osteogenic compositions exhibit improved osteoinductive properties, enhancing bone regeneration and healing processes by leveraging the synergistic effects of growth factors and viable osteogenic cells.
Implementation Method 1
The growth factors may be obtained from demineralized bone that has been contacted with an acidic extraction medium at a pH of between about 0.5 and about 4.4 at an extraction temperature between about 15° C. and 35° C. for between about 24 and 72 hours.
Implementation Method 2
the osteogenic composition may be cryopreserved
Implementation Method 3
the osteogenic composition may be lyopreserved
Data Source
AI summary
An osteogenic composition comprises growth-factor augmented cortical bone and cancellous bone having viable endogenous osteogenic cells that remain adherent to the cancellous bone. The growth-factor augmented cortical bone comprises demineralized cortical bone amplified with growth factors derived from a demineralized bone, and the cancellous bone not having been subjected to a loosening agent. The osteogenic composition is cryopreserved or lyopreserved. A method for preparing an enhanced graft composition with improved osteoinductive properties includes processing demineralized cortical bone; solubilizing growth factors from demineralized bone; and mixing the demineralized cortical bone with the solubilized growth factors.