Growth Factor Concentrate Preparation via Freeze-Drying
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Solution Overview
Problem
Current cosmetic treatments using platelet-rich plasma (PRP) face challenges due to the short shelf life of growth factors, low absorption rates, and the need for frequent venipuncture, as well as the use of additives to extend shelf life, which may not be effective.
Innovation Solution
A growth factor concentrate is developed by extracting PRP, stabilizing it with Tris Buffer, decapsulating the growth factors through freezing and thawing, filtering, and freeze-drying to create a stable, long-lasting product that can be stored for extended periods without additives.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Duration of action of stationary object
If PRP is used for growth factor treatment, then growth factors can be extracted from the subject, but the shelf life is limited to 5-9 days
Solution Approach 1:
The patent applies parameter changes by modifying the physical state of growth factors through freezing and thawing cycles, which alters their structural properties and enables long-term storage. The growth factors are frozen at -70°C to -80°C and then thawed, transforming them from a labile state to a stable state that can be stored for extended periods without degradation
Solution Approach 2:
The patent utilizes phase transitions of water (liquid to solid to vapor) through freeze-drying to preserve growth factors. The process involves freezing the PRP, removing water through sublimation under vacuum, and obtaining a stable powder form that can be stored indefinitely, thereby extending shelf life while maintaining biological activity
2Reliability
If growth factors are applied on skin surface, then cosmetic effects are achieved, but absorption is blocked by epidermis
Solution Approach 1:
The patent applies preliminary action by performing microneedling or laser treatment on the skin before applying the growth factor concentrate. This preliminary mechanical or thermal injury creates micro-channels in the epidermis that facilitate the penetration and absorption of growth factors into the deeper skin layers, overcoming the natural barrier function of the epidermis
3Productivity
If PRP treatment is performed multiple times over 3-6 months, then cumulative cosmetic effects are achieved, but frequent venipuncture is required
Solution Approach 1:
The patent applies preliminary action by extracting and processing growth factors from PRP in advance, then storing them in a stable, long-lasting form. This allows the growth factors to be readily available for multiple treatment sessions without requiring repeated venipuncture procedures, as the concentrated growth factors can be stored and reused
Solution Approach 2:
The patent creates a stable copy or replica of the active growth factors through freeze-drying and encapsulation processes. This preserved form maintains the biological activity and functional properties of the original growth factors while enabling long-term storage and repeated use, effectively copying their therapeutic potential without requiring continuous fresh extraction
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method results in a growth factor concentrate with a shelf life of at least 22 months, improved absorption rates, and reduced need for frequent venipuncture, providing effective skin rejuvenation and collagen synthesis without the use of preservatives.
Implementation Method 1
stabilizing the platelet rich plasma
Implementation Method 2
decapsulating the growth factors of the stabilized platelet rich plasma
Implementation Method 3
the decapsulating step further comprises the step of freezing and thawing the platelet rich plasma
Implementation Method 4
freeze-drying the filtered growth factors to obtain the growth factor concentrate
Data Source
AI summary
Provided herein are growth factor concentrates, the method of preparing the growth factor concentrates, cosmetic compositions and methods for cosmetic treatment. The growth factor concentrates include decapsulated growth factors derived from platelet rich plasma.