General-Purpose Growth Medium for Membrane Sterility Testing
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Solution Overview
Problem
Existing microbiological growth media, such as TSB and FTM, are inadequate for supporting the growth of aerobic and anaerobic bacteria, particularly on membranes, with FTM having limited growth promotion properties for many species and poor surface growth support for anaerobic organisms.
Innovation Solution
A composition comprising casein digest, soybean digest, animal tissue digest, yeast extract, dextrose, phosphate buffer, hemin, and L-cystine, optionally with a gelling agent and surfactant, supports the growth of anaerobes, molds, and general aerobic bacteria, providing a versatile medium for both solid and liquid forms.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If FTM is used to detect obligate anaerobes, then anaerobic bacteria can be grown, but the medium has limited growth promotion properties for many species and poor surface growth support
Solution Approach 1:
The patent combines the advantages of FTM (anaerobic detection capability) with TSB (general purpose growth promotion) by incorporating TSB powder into the FTM composition, creating a unified medium that supports both obligate anaerobes and a broader range of microorganisms effectively
Solution Approach 2:
The modified FTM medium achieves multi-functionality by simultaneously supporting the growth of obligate anaerobes, aerobes, and fastidious organisms, replacing the need for multiple separate media types while maintaining specialized anaerobic detection capabilities
2Adaptability or versatility
If TSB is used as a general purpose growth medium, then yeasts, molds, and aerobic bacteria can be detected, but it cannot effectively support anaerobic organism growth
Solution Approach 1:
The patent merges TSB's general purpose growth promotion capabilities with FTM's anaerobic support functions by incorporating TSB powder into the FTM formulation, creating a hybrid medium that delivers both aerobic and anaerobic growth support in a single system
3Reliability
If multiple separate media (TSB and FTM) are used for sterility testing, then comprehensive microbial detection is achieved, but the testing process becomes more complex and time-consuming
Solution Approach 1:
The patent combines multiple separate media functions into a single FTM-based composition by incorporating TSB powder, allowing comprehensive microbial detection (aerobes, anaerobes, fastidious organisms) to be achieved using one medium instead of multiple separate media systems
Solution Approach 2:
The modified FTM medium achieves universal applicability for sterility testing by simultaneously supporting the growth of diverse microorganisms including obligate anaerobes, aerobes, and fastidious organisms, replacing the need for multiple specialized media types
Data Source
AI summary
The present invention features general-purpose microbiological growth media capable of supporting growth of microorganisms on membranes. The media contain casein digest, soybean digest, animal tissue digest, yeast extract, dextrose, a phosphate buffer, hemin, and L-cystine. The invention features an all-purpose microbiological growth media that can support the growth of anaerobes, molds, injured spores, and general aerobic bacteria to a greater extent than other media.