GRP78 Peptide Screening to Exclude Senescent Stem Cells
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Solution Overview
Problem
Existing methods for developing stem cell therapy products using adult stem cells face challenges in screening out old cells due to long-term culture, leading to instability and ineffective quality control, as established markers are insufficient.
Innovation Solution
A GRP78-derived peptide with specific amino acid sequences is used to recognize and enhance the GRP78 protein, enabling the screening of highly efficient stem cells and increasing their efficiency by binding to the cell surface or being introduced into cells.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If adult stem cells are cultured for a long period to obtain sufficient cell numbers, then the quantity of stem cells increases, but the quality and efficiency of stem cells deteriorate due to accumulation of old cells and passage variations
Solution Approach 1:
The patent applies preliminary action by establishing a GRP78 peptide-based screening system before the stem cells deteriorate. The peptide marker is used to identify and isolate high-efficiency stem cells at early passages, preventing the accumulation of old, low-efficiency cells that would otherwise compromise product quality during long-term culture expansion
Solution Approach 2:
The GRP78-derived peptide acts as an intermediary marker that enables indirect detection of stem cell quality. Instead of directly measuring complex cellular functions, the peptide serves as a reliable surrogate marker that correlates with stem cell efficiency, allowing quality control through simplified detection methods
2Ease of operation
If established markers are used for quality control, then the ease of operation improves, but the measurement precision deteriorates because existing markers are insufficient to distinguish high-efficiency stem cells
Solution Approach 1:
The patent applies local quality by focusing on a specific molecular marker (GRP78 peptide) rather than relying on general, non-specific markers. This localized approach targets the particular quality attribute of stem cell efficiency, providing precise discrimination between high and low efficiency cells while maintaining operational simplicity through peptide-specific detection
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The peptide effectively screens and enhances the efficiency of adult stem cells, ensuring high differentiation and proliferation potential, thus improving the quality and efficacy of stem cell therapy products.
Implementation Method 1
a GRP78-derived peptide capable of binding to the binding domain of GRP78 protein on the cell surface
Data Source
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AI summary
The present invention relates to a GRP78-derived peptide for screening highly efficient stem cells and the use thereof, and more particularly, to screening highly efficient stem cells using, as a marker, a GRP78-derived peptide capable of binding to the binding domain of GRP78 protein on the cell surface. According to the present invention, the GRP78-derived peptide comprising only a specific amino acid sequence capable of recognizing highly efficient stem cells, among the amino acid sequence of GRP78, makes it possible to screen only non-senescent young stem cells. In addition, when stem cells are treated with the GRP78-derived peptide or the GRP78-derived peptide is introduced into stem cells, the efficiency of the stem cells can be increased. Thus, the GRP78-derived peptide is useful for the production of stem cell therapy products having excellent efficacy.