Gut Flora Fermentation Gas Detection With Gradient Injection
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current methods for detecting gut flora fermentation gases, particularly hydrogen, methane, carbon dioxide, and hydrogen sulfide, are inadequate due to the need for multiple detectors, manual injection leading to errors, and sensors that cannot handle the wide range of concentrations produced by gut flora, making comprehensive analysis difficult.
Innovation Solution
A small-scale batch fermentation system with a gradient injection method and customized gas analysis equipment that allows simultaneous detection of these gases using a single sensor, overcoming sensor range limitations and sample volume requirements.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If multiple detectors are used to detect different gases (hydrogen, methane, carbon dioxide, hydrogen sulfide), then detection comprehensiveness is improved, but device complexity increases
Solution Approach 1:
The patent employs a single gas chromatography detector that can detect multiple types of gases (hydrogen, methane, carbon dioxide, hydrogen sulfide) by using different detection methods and conditions. The detector is configured with multiple channels or modes that enable it to function as multiple specialized detectors would, thereby achieving comprehensive gas detection while maintaining a simple single-detector system.
2Ease of operation
If manual injection is used for gas sample analysis, then operation flexibility is improved, but measurement precision deteriorates due to injection errors
Solution Approach 1:
The gas chromatography system incorporates an automated injection mechanism that performs the sample introduction process without manual intervention. The system automatically handles gas sample injection, eliminating human error in the injection process while maintaining operational simplicity. The automation includes features such as automatic sample loading, precise injection timing, and consistent injection parameters, ensuring high precision and repeatability.
3Reliability
If sensors with fixed detection ranges are used, then sensor reliability is improved, but adaptability to varying gas concentrations deteriorates
Solution Approach 1:
The gas detection system employs sensors with adjustable detection ranges or multiple detection modes that can be dynamically selected based on the expected gas concentration levels. The system can switch between different sensor configurations or adjustment settings to accommodate varying concentration ranges, ensuring both reliability within each range and adaptability across different scenarios. This dynamic capability allows the same sensor system to reliably detect both low and high concentration gases by adjusting operational parameters.
4Measurement precision
If large sample volumes are required for gas detection, then detection accuracy is improved, but loss of substance increases
Solution Approach 1:
The system uses a small-volume gas chromatography method that creates an efficient analytical copy of the gas sample detection process. Instead of requiring large sample volumes for adequate detection, the system optimizes the detection process to work effectively with minimal sample amounts. The chromatography method is designed to maximize the information obtained from small samples through efficient separation and detection protocols, thereby achieving accurate results while minimizing sample consumption and loss.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables accurate, high-throughput, and automatic detection of gut flora fermentation gases, reducing sample volume needs and accommodating individual variations, providing comprehensive metabolic data for precision nutrition and medicine.
Implementation Method 1
a plurality of gas sensors and second air pressure sensors for a certain fermentation gas of gut flora are arranged in gas detection chamber 3
Implementation Method 2
the pressure of gas detection chamber reaching the second pressure P2 by using the vacuum generator connected to gas detection chamber, and then communicating the sample vial and the gas detection chamber to quantitatively extract the fermentation gas from the sample vial
Implementation Method 3
The pressure of sample vial reaching the first pressure P1 by using the air pump connected to sample vial
Implementation Method 4
a small batch fermentation system with a gradient injection method and customized gas analysis equipment that allows simultaneous detection of these gases
Data Source
AI summary
A method for testing gas produced by fermentation of intestinal flora includes placing a sample bottle into a sample bin; inserting the sampling needle into a sample bottle for sampling; communicating the sample bottle with a gas detecting cavity, and quantitatively extracting the fermentation gas from the sample bottle; detecting the concentration of a certain fermentation gas using a sensor to deduce the concentration of the fermentation gas in the sample bottle; repeating the detection by using a step-type injection mode until the detection in preset gradients of all the fermentation gas of gut flora is completed, and the detection data of each fermentation gas of gut flora conforms to the detection range of the preset gas sensor; and carrying out reduced calculation of the concentration of each fermentation gas of gut flora in the sample bottle according to the collected data. An instrument carries the test method.

