H. pylori Detection Primer Set for Saliva Sample Analysis
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Solution Overview
Problem
Current methods for detecting Helicobacter pylori (H. pylori) face challenges in efficiently isolating and culturing mucosal biopsy samples, and distinguishing H. pylori from other oral flora in saliva samples, which complicates early diagnosis and treatment of associated gastric diseases.
Innovation Solution
A primer set specifically designed to amplify target sequences of the cagA gene of H. pylori, allowing for the detection of H. pylori in saliva samples by amplifying nucleotide regions corresponding to positions 428-624, 433-668, or 431-671 of the cagA coding sequence, thereby differentiating it from other oral flora.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If mucosal biopsy samples are collected and cultured for H. pylori detection, then detection can be performed, but it is difficult to collect samples and H. pylori cannot be efficiently cultured due to irregular distribution and poor culturability
Solution Approach 1:
The patent replaces the mechanical/cultural system of biopsy collection and bacterial culture with a molecular detection system using PCR amplification of H. pylori-specific gene sequences. This substitution eliminates the need for difficult biopsy procedures and inefficient bacterial culture while maintaining high detection reliability through specific amplification of bacterial DNA from saliva samples.
2Ease of operation
If saliva samples are used for H. pylori detection, then sample collection becomes easier, but it is difficult to distinguish H. pylori from other oral flora
Solution Approach 1:
The patent applies local quality by designing primers that specifically target unique regions of the H. pylori genome (such as the urease gene or other species-specific sequences). This allows the detection system to distinguish H. pylori DNA from other oral bacteria in saliva samples by recognizing specific local genetic characteristics, thereby achieving high specificity while using easily collected saliva samples.
Solution Approach 2:
The patent uses H. pylori-specific gene sequences as intermediaries to bridge the gap between non-invasive saliva sampling and specific pathogen identification. These unique genetic markers serve as mediators that allow differentiation of H. pylori from other oral flora without requiring invasive procedures or complex culture methods.
3Loss of time
If conventional detection methods are used, then detection can be performed, but early stage H. pylori infection cannot be rapidly and reproducibly detected
Solution Approach 1:
The patent employs PCR amplification technology that can rapidly amplify even trace amounts of H. pylori DNA present in early-stage infections. This preliminary amplification action allows for the detection of low bacterial loads that would be missed by conventional methods, enabling early diagnosis while significantly reducing detection time through direct DNA analysis without requiring lengthy culture procedures.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The primer set enables efficient detection of H. pylori with high specificity, allowing for the use of saliva samples instead of biopsy samples, facilitating early diagnosis and treatment of gastric diseases.
Implementation Method 1
a primer set capable of amplifying at least one target sequence of the cagA gene of H. pylori
Data Source
AI summary
Provided are an oligonucleotide primer set for amplifying at least one target sequence of the cagA gene of Helicobacter pylori, a method of detecting Helicobacter pylori using the primer set, and a kit for detecting Helicobacter pylori, including the primer set.