Hyaluronic Acid Stabilized Autologous Cell Grafts
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Solution Overview
Problem
Current methods for treating tissue defects, such as wounds and bone fractures, face challenges in effectively delivering reparative cell populations without contamination risks and cell loss, particularly due to the need for culturing stem cells, which can introduce adventitious agents and result in the loss of non-adherent stem cells.
Innovation Solution
The use of hyaluronic acid (HA) or its derivatives (HAD) to stabilize autologous cell grafts, allowing for point-of-care generation and injection without culturing, combined with inductive media to promote differentiation into various cell types, providing a favorable environment for tissue repair.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If autologous cell populations are cultured before transplantation, then cell numbers can be increased, but contamination risks and cell loss increase due to adventitious agents
Solution Approach 1:
The patent applies preliminary action by pre-mixing autologous cell populations with hyaluronic acid or hyaluronic acid derivative at the point of care before transplantation, eliminating the need for subsequent culturing steps. This preliminary preparation allows the cells to be stabilized in a protective matrix that maintains viability while avoiding contamination risks associated with traditional culture methods
Solution Approach 2:
Hyaluronic acid or hyaluronic acid derivative serves as an intermediary substance that stabilizes and protects autologous cell populations during storage and transplantation. This intermediary matrix provides a protective environment that maintains cell viability without requiring traditional culture conditions, thereby preventing contamination while preserving cell functionality
2Quantity of substance
If traditional cell isolation methods are used, then cell populations can be obtained, but cell loss occurs due to non-adherent stem cells being discarded
Solution Approach 1:
The patent applies self-service by utilizing the natural properties of hyaluronic acid to automatically retain and stabilize all cell types including non-adherent stem cells. The hyaluronic acid matrix self-assembles around the cell population, providing inherent protection and stabilization without requiring additional handling steps that would cause cell loss
Solution Approach 2:
The patent changes the physical and chemical parameters of the cell storage environment by using hyaluronic acid or hyaluronic acid derivative instead of traditional culture media. This parameter change from liquid culture media to a gel-like hyaluronic acid matrix fundamentally alters cell behavior, allowing non-adherent cells to be retained and stabilized without requiring adherence to culture surfaces
3Quantity of substance
If cell culturing procedures are implemented, then cell proliferation can occur, but process complexity and time requirements increase
Solution Approach 1:
The patent extracts and eliminates the complex cell culturing procedures from the traditional cell transplantation process. By using hyaluronic acid to stabilize and protect autologous cell populations at the point of care, the method removes the need for separate culture chambers, media changes, and incubation protocols, thereby dramatically simplifying the overall process while maintaining cell viability
Solution Approach 2:
The patent skips the traditional cell culturing step entirely by using hyaluronic acid to stabilize and protect cells immediately upon isolation. This allows the process to rush through what would traditionally be a lengthy culturing phase, achieving cell proliferation and tissue repair benefits without the time and complexity constraints of conventional culture methods
Data Source
AI summary
Methods are described for generating autologous tissue grafts, including generating grafts at the point of care, which include isolated cell populations that are enriched with stem cells and are mixed with biological fillers including hyaluronic acid and derivatives thereof. The hyaluronic acid localizes the cells to a desired injection site and stimulates collagen production thus enhancing the viability and the longevity of the graft.