Patterned Flow Cell Preparation via Halogen Linker Chemistry
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Solution Overview
Problem
Existing gene sequencing methods are cumbersome and inefficient, particularly in the preparation of patterned flow cells, which increases time and economic costs, making it difficult to meet the needs of large-scale use.
Innovation Solution
A method for preparing a patterned flow cell involves providing a flow cell substrate with patterned binding regions, reacting a reaction reagent containing a halogen atom with these regions to form a linker, and then connecting a first nucleic acid to the linker to create the patterned flow cell.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional photolithography or printing technology is used to modify the target region, then the adapter can be adsorbed to capture the nucleic acid molecule, but the preparation steps become cumbersome and efficiency is low
Solution Approach 1:
The patent replaces conventional photolithography or printing technology with a chemical reaction-based approach. A reaction reagent containing a halogen atom reacts with the binding region to form a linker, which then connects to the nucleic acid. This chemical method simplifies the preparation steps and improves efficiency while maintaining the adapter adsorption capability.
Solution Approach 2:
The patent changes the chemical parameters of the binding region by introducing a halogen atom through chemical reaction. This modification enables the binding region to form a linker that can connect to nucleic acid molecules, thereby maintaining reliability while improving preparation efficiency.
2Reliability
If conventional methods are used to prepare patterned flow cell, then the adapter and primer can be attached to microsphere, but the time costs and economic costs increase
Solution Approach 1:
The patent replaces time-consuming conventional preparation methods with a streamlined chemical reaction process. The reaction reagent containing a halogen atom reacts with the binding region to form a linker that directly connects to nucleic acid, significantly reducing preparation time while maintaining microsphere loading capability.
Solution Approach 2:
The patent performs preliminary chemical modification of the binding region by introducing the halogen atom through chemical reaction before the actual microsphere loading. This preliminary action simplifies subsequent steps and reduces overall preparation time while ensuring reliable microsphere loading.
3Reliability
If complex preparation steps are used for patterned flow cell, then the binding region can be modified, but the economic costs increase
Solution Approach 1:
The patent replaces complex mechanical or chemical preparation steps with a simpler chemical reaction approach. The reaction reagent containing a halogen atom reacts with the binding region to form a linker that connects to nucleic acid, reducing manufacturing complexity and cost while maintaining reliable binding region modification.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method simplifies and enhances the efficiency of preparing patterned flow cells, allowing for rapid and convenient capture of microspheres, which facilitates high-throughput sequencing and reduces costs.
Implementation Method 1
a reaction reagent is reacted with the binding regions to obtain a substrate bound with a linker, wherein the linker contains a halogen atom from the reaction reagent
Implementation Method 2
the substrate bound is connected with the linker with a first nucleic acid to obtain a patterned flow cell
Data Source
AI summary
Provided are a patterned flow cell and a preparation method thereof, and a related sequencing method. The method for preparing a patterned flow cell includes: a) providing a flow cell substrate, wherein the flow cell substrate contains binding regions, and the binding regions are arranged in a patterned manner on the flow cell substrate; b) reacting a reaction reagent with the binding regions to obtain a substrate bound with a linker, wherein the linker contains a halogen atom from the reaction reagent; and c) connecting the substrate bound with the linker with a first nucleic acid to obtain a patterned flow cell.