Haploid-Inducer Corn Line AX6012 for Rapid Inbred Development

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Solution Overview

Problem

Traditional corn breeding methods are time-consuming and costly, involving multiple generations of inbreeding to produce homozygous lines with desired traits, and often result in unpredictable genetic variation and segregation, making it challenging to develop new inbred and hybrid corn varieties efficiently.

Innovation Solution

The development of a haploid-inducer corn line, AX6012, which generates haploid plants that can be doubled to produce highly uniform inbred lines, reducing the need for multigeneration inbreeding and allowing for more predictable genetic combinations, thereby accelerating the development of new inbred and hybrid corn varieties.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Stability of the object's composition

If traditional multigeneration inbreeding methods are used to produce homozygous lines, then genetic uniformity is achieved, but the breeding process becomes extremely time-consuming and costly

Engineering Contradiction:
Improvegenetic uniformityVSAvoidbreeding process duration
Core Design Contradiction:
Stability of the object's compositionVSLoss of time

Solution Approach 1:

The patent changes the ploidy parameter from diploid to haploid to achieve genetic uniformity in a single generation rather than requiring multiple generations of inbreeding. By inducing haploid plants that naturally self-doubt to become homozygous diploids, the breeding process is compressed from many generations to one, dramatically reducing time loss while maintaining genetic stability.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The patent introduces an intermediary mechanism (haploid induction system with color markers) that mediates between the parental diploid plants and the desired homozygous progeny. The haploid-inducer line AX6012 acts as an intermediary that facilitates rapid production of homozygous lines through controlled haploid formation and visual selection using color markers, eliminating the need for traditional prolonged inbreeding.

Inventive Principle:
Principle #24Intermediary (Mediator)

2Stability of the object's composition

If traditional inbreeding methods are used to develop new inbred corn lines, then homozygous lines are produced, but genetic variation becomes unpredictable and segregation occurs

Engineering Contradiction:
ImprovehomozygosityVSAvoidgenetic predictability
Core Design Contradiction:
Stability of the object's compositionVSLoss of information

Solution Approach 1:

The patent utilizes a parameter change in chromosome number (from diploid to haploid and back to homozygous diploid) to achieve predictable genetic outcomes. By inducing haploidy and subsequent self-doubling, each progeny plant is guaranteed to be completely homozygous with predictable inheritance of parental traits, eliminating the unpredictable segregation that occurs in traditional inbreeding where heterozygosity can persist across generations.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The patent employs color markers (visual phenotypic markers) to track and select for desired genetic combinations in the haploid-induced progeny. This allows breeders to visually identify and select plants with predictable inheritance patterns, maintaining genetic information integrity and enabling precise selection of homozygous lines with desired traits without the unpredictable segregation seen in traditional methods.

Inventive Principle:
Principle #32Color changes

3Manufacturing precision

If multiple generations of inbreeding are performed to produce superior inbred lines, then line uniformity is improved, but the cost and resource requirements increase significantly

Engineering Contradiction:
Improveline uniformityVSAvoidbreeding process complexity
Core Design Contradiction:
Manufacturing precisionVSEase of manufacture

Solution Approach 1:

The patent applies a parameter change (haploid induction) that produces completely uniform homozygous lines in a single generation, achieving the same line uniformity that would traditionally require multiple generations of inbreeding. This dramatically simplifies the breeding process by eliminating repeated crossing and selection cycles, reducing resource requirements while maintaining high line uniformity.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The patent introduces a haploid-inducer line with color markers as an intermediary system that simplifies the selection process. This intermediary mechanism provides visual identification of haploid plants and their progeny, enabling easy selection of uniform homozygous lines without complex multi-generational breeding protocols, thereby improving ease of manufacture while maintaining line uniformity.

Inventive Principle:
Principle #24Intermediary (Mediator)

Data Source

PatentUS8742237B1Haploid-inducer corn line designated AX6012
Publication Date: 2014.06.03 AGRIGENETICS INC

AI summary

A haploid-inducer corn line, designated AX6012, the plants and seeds of the haploid-inducer corn line AX6012, methods for producing a corn plant, either inbred or hybrid, produced through use of the haploid-inducer corn line AX6012 are disclosed. This invention also relates to methods for generating homozygous diploid corn plants from heterozygous diploid corn plants.