Hemoglobin A1C Analysis Kit Enzyme Stabilization

The patent changes the physical state of the enzyme reagent from liquid solution to dried powder form. This parameter change fundamentally improves thermal stability because the dried state reduces molecular mobility and prevents denaturation reactions that occur in liquid solutions at room temperature. The enzyme maintains its activity when dried and can be reconstituted when needed, solving the contradiction between measurement reliability and thermal stability.

The patent performs the drying action in advance during manufacturing, converting the reagent to a stable dried state for long-term storage. When needed, the user simply adds buffer solution to reconstitute the enzyme, which is a minimal preliminary action. This resolves the contradiction by preparing the reagent in its most stable form beforehand while maintaining ease of use through simple reconstitution.

The patent merges multiple reagents (hemolytic reagent, protease, FPOX, peroxidase, color developing reagent) into a single integrated cartridge system. This combination maintains measurement reliability through proper reagent formulation while dramatically reducing device complexity and improving portability. The single cartridge format eliminates the need for separate reagent storage and handling, making the system suitable for POCT applications.