HBsAg Detection Kit Using TCEP and Urea
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Solution Overview
Problem
Current HBsAg quantitative detection methods require sample dilution, which is time-consuming and reduces detection accuracy, as most clinical samples have HBsAg levels above the upper limit of detection for existing commercial reagents.
Innovation Solution
A kit and method using a reagent composition comprising tris(2-carboxyethyl)phosphine hydrochloride (TCEP) and urea, along with a monoclonal antibody, that allows for HBsAg detection up to 100,000 IU/mL without dilution, using a non-ionic surfactant, inorganic salt, and a buffer, simplifying the detection process.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If sample dilution is performed to detect HBsAg levels above the upper limit of detection, then the detection range is extended, but the detection time increases and detection accuracy decreases
Solution Approach 1:
The patent changes the chemical parameters of the detection reagent by incorporating TCEP (tris(2-carboxyethyl)phosphine hydrochloride) and urea, which modify the detection system's ability to handle high-concentration samples without dilution, thereby expanding the detection range while maintaining speed and accuracy
2Quantity of substance
If sample dilution is performed to detect HBsAg levels above the upper limit of detection, then the detection range is extended, but detection accuracy decreases
Solution Approach 1:
The patent modifies the reagent composition parameters by adding TCEP and urea, which improve the detection system's linearity and accuracy for high-concentration HBsAg samples, allowing accurate measurement without dilution-induced errors
3Quantity of substance
If sample dilution steps are added to detect high HBsAg levels, then the detection range increases, but the operational complexity increases
Solution Approach 1:
The patent extracts the dilution step from the detection process by developing a reagent system that can directly detect high-concentration samples, eliminating the need for preliminary sample dilution operations
4Ease of operation
If the upper limit of detection is increased to 100,000 IU/mL, then the need for dilution is eliminated, but the reagent composition complexity increases
Solution Approach 1:
The patent adjusts the chemical parameters of the reagent by incorporating specific concentrations of TCEP and urea, which enable the simplified detection process while the reagent complexity is managed through controlled composition specifications
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method ensures accurate detection of HBsAg levels within the range of most clinical samples without the need for dilution, improving detection efficiency and accuracy by expanding the upper limit of detection to 100,000 IU/mL.
Implementation Method 1
a first antibody capable of specifically binding to HBsAg
Implementation Method 2
the reagent composition comprising tris(2-carboxyethyl)phosphine hydrochloride (TCEP) and urea
Data Source
AI summary
A kit for quantitatively detecting HBsAg and a method for quantitatively detecting an HBsAg content in a sample containing HBsAg. The kit comprises a first antibody specifically binding to HBsAg and a reagent composition. The reagent composition comprises tris(2-carboxyethyl)phosphine hydrochloride (TCEP) and urea.