HBV Detection Probe Composition for NGS Genomic Insertion Site Analysis
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Solution Overview
Problem
Current methods for detecting hepatitis B virus (HBV) insertion sites across the entire human genome are limited, particularly in identifying potential liver cancer development sites, due to biases in traditional PCR-based approaches, which hinder comprehensive analysis.
Innovation Solution
A probe composition consisting of specific nucleotide sequences (SEQ ID NO: 1 to SEQ ID NO: 215) is used in conjunction with next-generation sequencing (NGS) to detect HBV insertion sites, optimizing probe length for high hybridization efficiency and providing almost 100% coverage of HBV genotypes, including a kit with reagents for PCR and sequencing.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If traditional PCR-based methods are used to detect HBV insertion sites, then detection specificity for localized virus regions is improved, but comprehensive coverage across the entire human genome is lost due to primer bias
Solution Approach 1:
The probe composition is divided into multiple individual probes (SEQ ID NO: 1 to SEQ ID NO: 215), each targeting specific HBV genomic regions. This segmentation allows comprehensive coverage of the entire HBV genome without relying on a single primer set, thereby achieving both specificity and comprehensive genome coverage simultaneously
Solution Approach 2:
The probe composition serves multiple functions: it detects various HBV genotypes (A through J), covers the entire HBV genome sequence, and identifies insertion sites across the human genome. This multi-functionality resolves the contradiction by providing universal detection capability while maintaining specific targeting through the hybridization mechanism
2Adaptability or versatility
If next-generation sequencing with probe composition is used, then comprehensive detection of HBV insertion sites across the entire human genome is improved, but detection cost increases
Solution Approach 1:
The probe composition uses synthesized nucleic acid sequences that are copies of the HBV genome regions to be detected. These probes are designed to be complementary to HBV sequences, allowing them to bind specifically to HBV insertion sites through hybridization, thereby enabling comprehensive detection without requiring expensive whole-genome sequencing of the entire human genome
Solution Approach 2:
The invention changes the detection parameter from sequencing the entire human genome to using targeted probe hybridization. By adjusting the probe design parameters (sequence specificity, length, and coverage), the method achieves comprehensive HBV detection at a lower cost compared to traditional whole-genome NGS approaches
3Measurement precision
If probe length is optimized for hybridization efficiency, then detection sensitivity is improved, but probe design complexity increases
Solution Approach 1:
The probe length parameter is optimized to balance hybridization efficiency with design feasibility. The probes are designed with appropriate lengths that ensure sufficient binding affinity for sensitive detection while remaining manageable for synthesis and analysis, thereby achieving high detection sensitivity without excessive design complexity
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables efficient and cost-effective detection of HBV insertion sites in the human genome, providing valuable information for liver cancer diagnosis and offering a more comprehensive analysis compared to traditional methods.
Implementation Method 1
hybridizing a target sample with a probe composition for detecting hepatitis B virus (HBV) consisting of a sequence selected from the group consisting of SEQ ID NO: 1 to SEQ ID NO: 215 to capture a target gene
Data Source
AI summary
A probe for detecting hepatitis B virus and a method for detecting an insertion site of hepatitis B virus at high efficiency based on the analysis method of next-generation sequencing using the probe is disclosed. A probe can be provided that is capable of confirming the insertion site of HBV in the human genome with a possibility of developing into liver cancer. In addition, by applying the probe to the analysis method of next-generation sequencing, HBV insertion sites in the human genome can be analyzed at low cost and high efficiency.