Helminth Egg Fraction Composition for Wound Healing

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Solution Overview

Problem

There is a need for new regenerative immunotherapies that can stimulate a type 2 immune response to promote wound healing and tissue repair without the pathologies associated with parasitic infections such as fibrosis and hepatic damage.

Innovation Solution

A regenerative composition comprising a mixture of a soluble fraction and an insoluble fraction derived from one or more helminth eggs, specifically produced by homogenizing helminth eggs, centrifuging to separate fractions, ultracentrifuging, filtering, and combining the soluble and insoluble fractions in a specific ratio to reduce proinflammatory responses.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Strength

If helminth eggs are used to stimulate type 2 immune response for wound healing, then tissue repair is promoted, but fibrosis and hepatic damage occur

Engineering Contradiction:

Improvetissue repairVSAvoidfibrosis and hepatic damage

Core Design Contradiction:

StrengthVSObject-affected harmful factors

Solution Approach 1:

The helminth egg preparation is segmented into multiple fractions through differential centrifugation: a soluble fraction containing proteins and a lipid fraction containing lipids. This segmentation allows selective use of beneficial components while excluding harmful ones, resolving the contradiction between promoting tissue repair and avoiding fibrosis

Inventive Principle:

Principle #1Segmentation

Solution Approach 2:

The patent extracts and removes the egg shell layer and other harmful components from the helminth egg preparation through centrifugation and filtration processes. Only the beneficial soluble and lipid fractions are retained for therapeutic use, eliminating the source of fibrosis and hepatic damage while preserving tissue repair capabilities

Inventive Principle:

Principle #2Taking out (Extraction)

2Reliability

If helminth infection is used to reduce allergies and modulate immunity, then beneficial immunomodulation occurs, but parasitic infection pathologies arise

Engineering Contradiction:

ImproveimmunomodulationVSAvoidparasitic infection pathologies

Core Design Contradiction:

ReliabilityVSObject-generated harmful factors

Solution Approach 1:

The patent uses purified helminth egg fractions (soluble and lipid) as intermediary substances that can be administered without causing actual parasitic infection. These fractions serve as mediators that trigger beneficial type 2 immune responses and immunomodulation while avoiding the harmful effects of live parasitic worms and complete egg infections

Inventive Principle:

Principle #24Intermediary (Mediator)

3Object-affected harmful factors

If soluble fraction and insoluble fraction are combined in specific ratio, then proinflammatory response is reduced, but formulation complexity increases

Engineering Contradiction:

Improveproinflammatory responseVSAvoidformulation complexity

Core Design Contradiction:

Object-affected harmful factorsVSDevice complexity

Solution Approach 1:

The patent optimizes the ratio of soluble fraction to insoluble fraction (lipid fraction) through systematic parameter adjustment during formulation. By controlling the proportion of these fractions, the proinflammatory response is minimized while maintaining tissue repair benefits, and the formulation process remains manageable through defined centrifugation and mixing parameters

Inventive Principle:

Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The composition stimulates a type 2 immune response, promoting wound healing and tissue repair by increasing IL-4 levels and decreasing IL-17 levels, while minimizing scar tissue and fibrotic tissue formation.

Implementation Method 1

homogenizing one or more helminth eggs

Methodology Applied

Scientific EffectHomogenization:

Implementation Method 2

centrifuging the homogenized helminth eggs to form an insoluble fraction, a soluble fraction, and an egg shell fraction

Methodology Applied

Scientific EffectCentrifugal separation: Centrifugal Separation

Implementation Method 3

ultracentrifuging the soluble fraction and insoluble fraction

Methodology Applied

Scientific EffectUltracentrifugation:

Implementation Method 4

filtering each of the soluble and insoluble layers separately

Methodology Applied

Scientific EffectFiltration: Filter (physical)

Data Source

PatentUS20250161371A1Regenerative helminth containing compositions and uses thereof

Publication Date: 2025.05.22 JOHNS HOPKINS UNIVERSITY

AI summary

Disclosed herein are compositions containing a mixture of an soluble fraction and insoluble fraction derived from at least one helminth egg for use in treating wounds and/or injuries to tissues in subjects in need of treatment thereof.