Hemoglobin Stabilization via Acetylacetone and Uric Acid
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Solution Overview
Problem
Hemoglobin has low preservation stability in solution, leading to denaturation and decomposition, which affects the accuracy of quality control in laboratory tests, particularly in urine, blood, and saliva testing.
Innovation Solution
Incorporating acetylacetone and uric acid into the hemoglobin-containing solution to stabilize hemoglobin, with concentrations of acetylacetone ranging from 10 mg/dL to 200 mg/dL and uric acid from 1 mg/dL to 20 mg/dL, allowing for prolonged preservation at various temperatures.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Stability of the object's composition
If hemoglobin is preserved in solution without stabilizers, then the solution is simple and easy to prepare, but hemoglobin undergoes denaturation and decomposition leading to low preservation stability
Solution Approach 1:
The patent introduces acetylacetone and uric acid as intermediary substances that mediate between hemoglobin and the aqueous environment. These stabilizers form protective complexes with hemoglobin, preventing direct interaction with denaturing agents in the solution, thereby maintaining hemoglobin stability without requiring complex formulation systems
Solution Approach 2:
The patent optimizes specific concentration parameters of acetylacetone (1-20 mM) and uric acid (0.1-2 mM) to achieve optimal hemoglobin stability. By precisely controlling these chemical parameter ranges, the solution maintains hemoglobin integrity without requiring excessive stabilizer concentrations or complex compositional adjustments
2Duration of action of stationary object
If hemoglobin is preserved in solution with stabilizers, then preservation stability is improved, but the cost and complexity of reagent preparation increase
Solution Approach 1:
The patent employs acetylacetone and uric acid as inexpensive, readily available stabilizing agents that can be easily obtained and incorporated into the solution. These simple, low-cost additives extend the usable life of hemoglobin solutions without requiring expensive specialized stabilizers or complex manufacturing procedures
Solution Approach 2:
The patent creates a composite stabilizing system combining acetylacetone and uric acid in specific ratios within the hemoglobin solution. This composite approach leverages the synergistic effects of both substances to achieve extended preservation periods while maintaining straightforward preparation methods using common chemical reagents
3Reliability
If hemoglobin solution is used for quality control testing, then measurement accuracy is maintained, but hemoglobin decomposition affects the reliability of control values over time
Solution Approach 1:
The patent applies preliminary stabilization treatment by incorporating acetylacetone and uric acid into the hemoglobin solution before use. This pre-established protective environment prevents decomposition during storage and handling, ensuring that quality control measurements remain reliable throughout the intended usage period without requiring frequent re-preparation of control solutions
Data Source
AI summary
A method that is capable of stably preserving hemoglobin in a solution. A method for stabilizing hemoglobin in a solution includes causing at least one of acetylacetone or uric acid to coexist in the solution containing hemoglobin.