Capillary Electrophoresis Hemoglobin Analysis via Dual-Parameter Detection
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Solution Overview
Problem
Existing methods for analyzing hemoglobin species, such as HbA1c and mutant Hb, face challenges in specifying components when peak overlap occurs due to varying concentrations, making it difficult to accurately identify Hb species in samples.
Innovation Solution
A sample analysis method using capillary electrophoresis to separate components, acquire electropherograms, and specify components based on a correlation between detection time and concentration, employing parameters like slope data or boundary lines to correct detection times and identify Hb species regardless of concentration.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If capillary electrophoresis is used to separate hemoglobin components, then the apparatus can be downsized and measurement time can be shortened, but peak overlap occurs when hemoglobin concentration varies, making it difficult to specify Hb species
Solution Approach 1:
The invention transitions from one-dimensional detection (time only) to two-dimensional detection by introducing absorbance as a second parameter. By plotting detection time against absorbance, the method creates a two-dimensional parameter space that resolves peak overlaps occurring in the time dimension alone, enabling accurate component specification even when peaks co-elute
Solution Approach 2:
The invention changes the detection parameters from relying solely on detection time to using both detection time and absorbance. By calculating absorbance at detected time points and using these dual parameters for component identification, the method overcomes the limitation of time-based identification that fails when peaks overlap due to concentration variations
2Device complexity
If simple apparatus is used for hemoglobin analysis, then apparatus size is reduced, but it becomes difficult to specify Hb species when peaks overlap due to concentration variations
Solution Approach 1:
The invention adds a second detection dimension (absorbance) to the simple capillary electrophoresis apparatus. By measuring both detection time and absorbance, the system achieves accurate component specification without requiring complex additional hardware, maintaining apparatus simplicity while improving measurement capability
Solution Approach 2:
The invention enhances the analytical capability by changing from single-parameter (time) detection to dual-parameter (time and absorbance) detection. This parameter expansion allows the simple apparatus to accurately identify Hb species even in challenging overlap conditions
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables accurate specification of hemoglobin species by correcting detection times using correlation parameters, effectively addressing the issue of peak overlap and concentration variability, thereby improving analysis precision.
Implementation Method 1
a step of separating a plurality of components included in a sample by capillary electrophoresis
Implementation Method 2
a step of optically measuring the sample to obtain an absorbance
Data Source
AI summary
Provided are a sample analysis method and a sample analysis apparatus capable of specifying a component included in a sample regardless of a concentration of the component included in the sample. A sample analysis method or the like includes a step of separating plural components included in a sample by capillary electrophoresis; a step of obtaining an electropherogram of the sample; a step of acquiring a detection time of each peak and a concentration corresponding to each peak from the electropherogram; and a step of specifying a component included in the sample using a parameter indicating a correlation between a detection time and a concentration calculated using a known component with respect to the acquired detection time and the acquired concentration.