Hemolysis Platelet Detection via Membrane Fluorescence
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Solution Overview
Problem
Current methods for detecting platelets in blood samples are inaccurate under hemolysis conditions, particularly when faced with special samples containing large platelets and small red blood cells, and require separate channels, which complicates instruments and increases costs.
Innovation Solution
A reagent comprising a hemolytic agent and a membrane-specific or mitochondrion-specific dye is used to treat blood samples, allowing for the differentiation and counting of platelets under hemolysis conditions in a single channel, along with the classification and counting of white blood cells and detection of reticulocytes.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If electrical impedance method is used for platelet detection, then measurement speed is fast, but measurement precision deteriorates when facing special samples with large platelets and small red blood cells
Solution Approach 1:
The patent changes the detection parameter from electrical impedance to optical fluorescence detection. By using a membrane-specific dye that binds to platelet membranes and emits fluorescence, the system can accurately detect platelets based on their fluorescent signal rather than electrical properties, thereby resolving the accuracy issue with special samples while maintaining fast measurement speed
Solution Approach 2:
The patent replaces the electrical impedance measurement system with an optical detection system. Instead of measuring electrical resistance changes, the system uses fluorescence emission from dyed platelet membranes, substituting mechanical/electrical measurement with optical measurement to achieve better precision for special samples
2Measurement precision
If labeled antibody method is used for platelet detection, then measurement precision is improved, but measurement time increases significantly
Solution Approach 1:
The patent uses a membrane-specific dye that can be rapidly bound to platelet membranes without requiring time-consuming antigen-antibody reactions. The dye directly stains the platelet membrane, providing quick and accurate detection, thus replacing the time-consuming labeled antibody method while maintaining precision
Solution Approach 2:
The patent performs preliminary membrane staining with the specific dye during the same measurement process, eliminating the need for separate labeling steps. By preparing the platelets for detection in advance through rapid membrane binding, the system achieves both speed and accuracy
3Measurement precision
If separate channels are used for platelet analysis, then measurement precision is maintained, but device complexity increases
Solution Approach 1:
The patent merges platelet detection with white blood cell classification into a single detection channel. By using a membrane-specific dye that stains both platelet membranes and white blood cell membranes, the system can detect and differentiate these cell types simultaneously in one channel, thereby reducing instrument complexity while maintaining precision
Solution Approach 2:
The patent makes the detection system universal by using a single membrane-specific dye that can stain multiple cell types (platelets and white blood cells). This multi-functional dye allows one detection channel to perform multiple functions (platelet counting and white blood cell classification), eliminating the need for separate dedicated channels
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enables quick and accurate detection of platelets, reduces instrument complexity, and simplifies the analysis process by eliminating the need for separate channels, while providing simultaneous analysis of platelets, white blood cells, and reticulocytes.
Implementation Method 1
a second reagent which is a membrane-specific dye or a mitochondrion-specific dye
Data Source
AI summary
A reagent, a method for differentiating platelets under a hemolysis condition using the reagent and a blood cell analyzer are provided. The reagent includes a first reagent as a hemolytic agent, and a second reagent which is a membrane-specific dye or a mitochondrion-specific dye. By using the reagent, the method and the blood cell analyzer, platelets can be differentiated and an alarm about reticulocytes can be provided under a hemolysis condition, and white blood cell can further be classified and counted, thereby quickly and accurately analyzing a blood sample in a single channel.