Herbicide Tolerance Protein Mutations for Sulfonylurea Resistance
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Solution Overview
Problem
The emergence of glyphosate-resistant weeds and the expanding use of sulfonylurea herbicides necessitate the development of genes capable of degrading sulfonylurea herbicides to enhance plant tolerance, as existing herbicides are becoming less effective against difficult-to-control weed species.
Innovation Solution
Introduction of a herbicide tolerant protein with specific amino acid substitutions, such as alanine and valine substitutions at designated positions, and a nucleotide sequence encoding this protein, which enhances sulfonylurea herbicide degradation and tolerance in plants.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If sulfonylurea herbicides are used to control glyphosate-resistant weeds, then weed control effectiveness is improved, but plant damage from herbicides increases
Solution Approach 1:
The patent introduces specific amino acid substitutions (alanine at position 176 and/or valine at position 178 in SEQ ID NO:1) in the ALS protein to change the molecular structure parameters, enabling the plant's ALS enzyme to resist sulfonylurea herbicide inhibition while maintaining its physiological function, thus allowing effective weed control without plant damage
Solution Approach 2:
The patent converts the harmful effect of sulfonylurea herbicides on the ALS enzyme into a benefit by introducing specific mutations that make the ALS protein resistant to herbicide binding, while the herbicides continue to effectively kill susceptible weeds. This allows the same herbicide to be both a weapon against weeds and safe for the crop
2Productivity
If existing ALS proteins are used in plants, then plants can perform normal amino acid synthesis, but plants are highly sensitive to sulfonylurea herbicides
Solution Approach 1:
The patent modifies the ALS protein sequence by introducing specific amino acid substitutions (alanine at position 176 and/or valine at position 178) to change the binding site characteristics, reducing herbicide affinity while preserving the enzyme's catalytic activity for amino acid synthesis
Solution Approach 2:
The patent applies local quality modification by making specific changes only at positions 176 and 178 of the ALS protein sequence, leaving the rest of the enzyme structure unchanged to maintain its physiological function while conferring herbicide resistance at the critical binding region
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The herbicide tolerant protein and gene enable plants to withstand higher concentrations of sulfonylurea herbicides, providing effective weed control and increased crop yields by reducing damage from these herbicides.
Implementation Method 1
the herbicide tolerant protein is capable of better degrading sulfonylurea herbicides
Data Source
AI summary
The present invention relates to an herbicide tolerance protein, an encoding gene thereof and use thereof, the herbicide tolerance protein comprising: a protein (a) having an amino acid sequence as shown in SEQ ID NO: 1, and having an alanine substitution at least at position 176 and/or having a valine substitution at position 178 of SEQ ID NO: 1; or (b) having an amino acid sequence as shown in SEQ ID NO: 3; or (c) having an amino acid sequence as shown in SEQ ID NO: 5; or (d) having an amino acid sequence as shown in SEQ ID NO: 7; or (e) being derived from (a) by means of the amino acid sequence of (a) undergoing substitution and/or deletion and/or by added one or several amino acids, and having the activity of thifensulfuron hydrolase. The herbicide tolerance protein of the present invention has a broad application prospects in plants.