Hericium Erinaceus HE015 Strain Selection and Molecular Identification
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Solution Overview
Problem
The existing Hericium erinaceus strains face issues of low genetic diversity, high yield versus quality contradictions, strain vitality degeneration, and living contaminants, which hinder the cultivation industry and result in economic losses.
Innovation Solution
A new Hericium erinaceus strain HE015 is developed through systematic breeding from wild samples, characterized by dense mycelia, fast growth, high yield, short growth cycle, and strong resistance to living contaminants, along with a specific molecular marker for identification and a polysaccharide extract for gastric mucosal protection.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If traditional mutation breeding and crossbreeding methods are used to develop new Hericium erinaceus varieties, then new varieties can be obtained, but the genetic background remains similar and the varieties show little differentiation in characteristics
Solution Approach 1:
Instead of using traditional mutation breeding that starts from existing strains, this patent inverts the approach by directly isolating and screening wild-type strains from natural environments. This inversion allows access to entirely new genetic backgrounds that have not been subjected to artificial selection or mutation, thereby achieving both high genetic diversity and significant variety differentiation.
Solution Approach 2:
The patent introduces molecular marker technology as an intermediary tool to identify and characterize wild-type strains. By using molecular markers, researchers can efficiently screen and differentiate strains based on their genetic profiles, enabling the selection of strains with novel genetic backgrounds and characteristics without relying on traditional breeding methods.
2Productivity
If breeding focuses on high yield characteristics, then yield increases, but quality and fine mushroom shape deteriorate
Solution Approach 1:
The patent changes the selection parameters from traditional yield-focused criteria to a multi-parameter screening system that includes molecular marker profiles, growth rate, contamination resistance, and quality characteristics. This parameter change enables the identification of wild-type strains that naturally possess both high yield potential and superior quality traits, resolving the contradiction between yield and quality.
Solution Approach 2:
The patent develops a comprehensive evaluation system that simultaneously assesses multiple functions and characteristics of strains, including productivity, quality, resistance, and genetic uniqueness. This multi-functional approach allows the selection of strains that excel across multiple parameters, rather than optimizing for yield alone, thereby achieving both high yield and fine quality.
3Productivity
If traditional strains are used for scale production, then production can be maintained, but strain vitality degenerates and living contaminants increase, causing economic losses
Solution Approach 1:
The patent applies preliminary screening and selection of wild-type strains with superior vitality and contamination resistance before scale production. By pre-selecting strains with robust genetic backgrounds and resistance traits using molecular markers and controlled trials, the patent prevents vitality degradation and contamination issues before they occur in production, ensuring long-term production stability.
Solution Approach 2:
The patent implements a feedback mechanism through molecular marker monitoring and performance tracking during production. By continuously monitoring strain characteristics and comparing them against baseline wild-type profiles, the system can detect early signs of vitality degradation or contamination and take corrective actions, thereby maintaining reliable production over time.
Data Source
AI summary
The present invention discloses a new Hericium erinaceus strain HE015, and a molecular marker and use thereof. Wild fungi are widely collected throughout China and subjected to systematic strain breeding to obtain a new fine-quality Hericium erinaceus strain HE015. The strain HE015 was preserved in Guangdong Microbial Culture Collection Center on May 12, 2022 with an accession number of GDMCC No: 62464. The genetic background of the strain is obviously different from that of the existing Hericium erinaceus species; therefore, the new strain is of important value and significance in supplementing fine-quality cultivated Hericium erinaceus varieties, meeting the market demand and promoting high-quality development of the industry. The present invention further provides a specific molecular marker and a rapid detection method for detecting and identifying the strain HE015.